Y Yang1, N Xun, J-G Wu. 1. Department of Pathology, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, China. ftjaxh@163.com.
Abstract
OBJECTIVE: The heterogeneity of breast cancer leads to its complexity and diversity in the process of evolution, which brings great difficulties to the stratification and individualized treatment of breast cancer patients. The long noncoding RNA FGF14 antisense RNA 2 (FGF14-AS2) is concerned with the progression and prognosis of breast cancer, but the underlying molecular mechanism of FGF14-AS2 in breast cancer has rarely been reported. PATIENTS AND METHODS: The expressions of FGF14-AS2 and miR-205-5p in breast cancer tissues and cells were detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The proliferation, migration, invasion, and apoptosis of breast cells were assessed by MTT or transwell or flow cytometry assay. The interaction between FGF14-AS2 and miR-205-5p were predicted by lncRNA-microRNA database DIANA-LncBase v2 and confirmed by the Dual-Luciferase Reporter Assay System. RESULTS: FGF14-AS2 was down-regulated while miR-205-5p was up-regulated in breast cancer tissues and cells and correlated with tumor stage and size. Functionally, the overexpression of FGF14-AS2 or miR-205-5p knockdown suppressed proliferation, migration, and invasion, and induced apoptosis of breast cancer cells. Moreover, FGF14-AS2 could directly bind to miR-205-5p, and the overexpression of FGF14-AS2 undermined the miR-205-5p induced effects on proliferation, migration, invasion, and apoptosis in breast cancer cells. CONCLUSIONS: FGF14-AS2 directly bind to miR-205-5p to repress proliferation, migration, invasion, and induce apoptosis in breast cancer. This study may provide a potential therapeutic strategy for breast cancer.
OBJECTIVE: The heterogeneity of breast cancer leads to its complexity and diversity in the process of evolution, which brings great difficulties to the stratification and individualized treatment of breast cancerpatients. The long noncoding RNA FGF14 antisense RNA 2 (FGF14-AS2) is concerned with the progression and prognosis of breast cancer, but the underlying molecular mechanism of FGF14-AS2 in breast cancer has rarely been reported. PATIENTS AND METHODS: The expressions of FGF14-AS2 and miR-205-5p in breast cancer tissues and cells were detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The proliferation, migration, invasion, and apoptosis of breast cells were assessed by MTT or transwell or flow cytometry assay. The interaction between FGF14-AS2 and miR-205-5p were predicted by lncRNA-microRNA database DIANA-LncBase v2 and confirmed by the Dual-Luciferase Reporter Assay System. RESULTS:FGF14-AS2 was down-regulated while miR-205-5p was up-regulated in breast cancer tissues and cells and correlated with tumor stage and size. Functionally, the overexpression of FGF14-AS2 or miR-205-5p knockdown suppressed proliferation, migration, and invasion, and induced apoptosis of breast cancer cells. Moreover, FGF14-AS2 could directly bind to miR-205-5p, and the overexpression of FGF14-AS2 undermined the miR-205-5p induced effects on proliferation, migration, invasion, and apoptosis in breast cancer cells. CONCLUSIONS:FGF14-AS2 directly bind to miR-205-5p to repress proliferation, migration, invasion, and induce apoptosis in breast cancer. This study may provide a potential therapeutic strategy for breast cancer.
Authors: Michele Salemi; Giuseppe Lanza; Maria Paola Mogavero; Filomena I I Cosentino; Eugenia Borgione; Roberta Iorio; Giovanna Maria Ventola; Giovanna Marchese; Maria Grazia Salluzzo; Maria Ravo; Raffaele Ferri Journal: Int J Mol Sci Date: 2022-01-28 Impact factor: 5.923