Mubin Mustafa Kiyani1, Maisra Azhar Butt2, Hamza Rehman3, Hussain Ali4, Syed Ali Hussain5, Sumaiyah Obaid5, Mir Arif Hussain5, Tariq Mahmood6, Syed Ali Imran Bokhari7. 1. Department of Bioinformatics and Biotechnology, Faculty of Basic and Applied sciences, International Islamic university Islamabad, Pakistan; Faculty of Rehabilitation and Allied Health Sciences, Riphah International University, Islamabad, Pakistan. 2. Department of animal sciences, Quaid-i-Azam University, Islamabad, Pakistan. 3. Department of Bioinformatics and Biotechnology, Faculty of Basic and Applied sciences, International Islamic university Islamabad, Pakistan. Electronic address: hamzarehman51@gmail.com. 4. Veterinary Farms Management, National Institute of Health, Islamabad, Pakistan. 5. Faculty of Rehabilitation and Allied Health Sciences, Riphah International University, Islamabad, Pakistan. 6. Department of Nanoscience and Technology, National Center for Physics, Islamabad, Pakistan. 7. Department of Bioinformatics and Biotechnology, Faculty of Basic and Applied sciences, International Islamic university Islamabad, Pakistan.
Abstract
BACKGROUND: Zinc is an essential trace element which is involved in controlling oxidative stress, growth and immune system by regulating inflammatory cytokines. Gouty arthritis is the inflammation of joints and tissues caused by the accumulation of monosodium urate crystals. METHOD AND OBJECTIVE: This study involved the oral administration of zinc oxide nanoparticles at a various concentration (5 ppm, 10 ppm, and 20 ppm) and study their antioxidant and anti-gout effects on Balb/C mice. Various parameters such as ROS, superoxide, peroxide, catalase, TBARS, RFTs, LFTs, lipid profile and blood count were studied. RESULTS: ZnO nanoparticles at the concentrations of 10 and 20 ppm were significant (P < 0.001) in reducing serum uric acid concentration thus treating gouty arthritis. Reactive oxygen species and thiobarbituric acid reactive substances were significantly increased in comparison to zinc oxide nanoparticles treated groups. Furthermore, blood count and LFTs also showed the effectiveness of zinc oxide in the reduction of hyperuricemia. Histopathological analysis showed no apparent changes in liver, kidney and muscles tissues. CONCLUSION: Zinc oxide nanoparticles can be effective in reducing oxidative stress and the treatment of gouty arthritis.
BACKGROUND: Zinc is an essential trace element which is involved in controlling oxidative stress, growth and immune system by regulating inflammatory cytokines. Gouty arthritis is the inflammation of joints and tissues caused by the accumulation of monosodium urate crystals. METHOD AND OBJECTIVE: This study involved the oral administration of zinc oxide nanoparticles at a various concentration (5 ppm, 10 ppm, and 20 ppm) and study their antioxidant and anti-gout effects on Balb/C mice. Various parameters such as ROS, superoxide, peroxide, catalase, TBARS, RFTs, LFTs, lipid profile and blood count were studied. RESULTS: ZnO nanoparticles at the concentrations of 10 and 20 ppm were significant (P < 0.001) in reducing serum uric acid concentration thus treating gouty arthritis. Reactive oxygen species and thiobarbituric acid reactive substances were significantly increased in comparison to zinc oxide nanoparticles treated groups. Furthermore, blood count and LFTs also showed the effectiveness of zinc oxide in the reduction of hyperuricemia. Histopathological analysis showed no apparent changes in liver, kidney and muscles tissues. CONCLUSION: Zinc oxide nanoparticles can be effective in reducing oxidative stress and the treatment of gouty arthritis.
Authors: Muhammad Riaz; Lina Tariq Al Kury; Noreen Atzaz; Abdullah Alattar; Reem Alshaman; Fawad Ali Shah; Shupeng Li Journal: Drug Des Devel Ther Date: 2022-04-22 Impact factor: 4.319