Literature DB >> 31478642

Naked-Eye Detection of Grapevine Red-Blotch Viral Infection Using a Plasmonic CRISPR Cas12a Assay.

Yongya Li1,2, Hayam Mansour2,3, Tony Wang4, Sudarsana Poojari4, Feng Li1,2.   

Abstract

Herein, we described a novel plasmonic CRISPR Cas12a assay for the visual, colorimetric detection of grapevine viral infections. Our assay generates rapid and specific colorimetric signals for nucleic acid amplicons by combining the unique target-induced incriminate single-stranded DNase activity of Cas12a with plasmon coupling of DNA functionalized gold nanoparticles. The practical applicability of our plasmonic assay was successfully demonstrated through the detection of emerging red-blotch viral infections in grapevine samples collected from commercial vineyards.

Entities:  

Year:  2019        PMID: 31478642     DOI: 10.1021/acs.analchem.9b03545

Source DB:  PubMed          Journal:  Anal Chem        ISSN: 0003-2700            Impact factor:   6.986


  15 in total

1.  Probing CRISPR-Cas12a Nuclease Activity Using Double-Stranded DNA-Templated Fluorescent Substrates.

Authors:  Christopher W Smith; Nidhi Nandu; Mahera J Kachwala; Yu-Sheng Chen; Taha Bilal Uyar; Mehmet V Yigit
Journal:  Biochemistry       Date:  2020-04-07       Impact factor: 3.162

2.  Recognition of DNA Target Formulations by CRISPR-Cas12a Using a dsDNA Reporter.

Authors:  Christopher W Smith; Mahera J Kachwala; Nidhi Nandu; Mehmet V Yigit
Journal:  ACS Synth Biol       Date:  2021-06-18       Impact factor: 5.249

3.  Detection of Four Porcine Enteric Coronaviruses Using CRISPR-Cas12a Combined with Multiplex Reverse Transcriptase Loop-Mediated Isothermal Amplification Assay.

Authors:  Jiajia Liu; Dagang Tao; Xinquan Chen; Linyuan Shen; Li Zhu; Bingrong Xu; Hailong Liu; Shuhong Zhao; Xinyun Li; Xiangdong Liu; Shengsong Xie; Lili Niu
Journal:  Viruses       Date:  2022-04-17       Impact factor: 5.048

4.  Cas12a-Activated Universal Field-Deployable Detectors for Bacterial Diagnostics.

Authors:  Yun Sun; Hong Liu; Yuanyuan Shen; Xingmei Huang; Fengge Song; Xiaolin Ge; Aimin Wang; Kaixiang Zhang; Yue Li; Chaoyang Li; Yi Wan; Jinghong Li
Journal:  ACS Omega       Date:  2020-06-08

Review 5.  Next-generation pathogen diagnosis with CRISPR/Cas-based detection methods.

Authors:  Xinjie Wang; Xiaoyun Shang; Xingxu Huang
Journal:  Emerg Microbes Infect       Date:  2020-12       Impact factor: 7.163

6.  Field detection of multiple RNA viruses/viroids in apple using a CRISPR/Cas12a-based visual assay.

Authors:  Jian Jiao; Kangkang Kong; Jinmeng Han; Shangwei Song; Tuanhui Bai; Chunhui Song; Miaomiao Wang; Zhenli Yan; Hengtao Zhang; Ruiping Zhang; Jiancan Feng; Xianbo Zheng
Journal:  Plant Biotechnol J       Date:  2020-09-17       Impact factor: 9.803

Review 7.  Potential of CRISPR/Cas system in the diagnosis of COVID-19 infection.

Authors:  V Edwin Hillary; Savarimuthu Ignacimuthu; S Antony Ceasar
Journal:  Expert Rev Mol Diagn       Date:  2021-11-09       Impact factor: 5.225

8.  CRISPR-based DNA and RNA detection with liquid-liquid phase separation.

Authors:  Willem Kasper Spoelstra; Jeroen M Jacques; Rodrigo Gonzalez-Linares; Franklin L Nobrega; Anna C Haagsma; Marileen Dogterom; Dimphna H Meijer; Timon Idema; Stan J J Brouns; Louis Reese
Journal:  Biophys J       Date:  2021-02-20       Impact factor: 4.033

9.  An ultrasensitive hybridization chain reaction-amplified CRISPR-Cas12a aptasensor for extracellular vesicle surface protein quantification.

Authors:  Shan Xing; Zedong Lu; Qi Huang; Huilan Li; Yu Wang; Yanzhen Lai; Yi He; Min Deng; Wanli Liu
Journal:  Theranostics       Date:  2020-08-13       Impact factor: 11.556

Review 10.  Programmable Biosensors Based on RNA-Guided CRISPR/Cas Endonuclease.

Authors:  Xiaolong Liu; Mubashir Hussain; Jianguo Dai; Yonghong Li; Lijun Zhang; Jian Yang; Zeeshan Ali; Nongyue He; Yongjun Tang
Journal:  Biol Proced Online       Date:  2022-01-23       Impact factor: 3.244

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