| Literature DB >> 31478642 |
Yongya Li1,2, Hayam Mansour2,3, Tony Wang4, Sudarsana Poojari4, Feng Li1,2.
Abstract
Herein, we described a novel plasmonic CRISPR Cas12a assay for the visual, colorimetric detection of grapevine viral infections. Our assay generates rapid and specific colorimetric signals for nucleic acid amplicons by combining the unique target-induced incriminate single-stranded DNase activity of Cas12a with plasmon coupling of DNA functionalized gold nanoparticles. The practical applicability of our plasmonic assay was successfully demonstrated through the detection of emerging red-blotch viral infections in grapevine samples collected from commercial vineyards.Entities:
Year: 2019 PMID: 31478642 DOI: 10.1021/acs.analchem.9b03545
Source DB: PubMed Journal: Anal Chem ISSN: 0003-2700 Impact factor: 6.986