Elsa Almeida1, Elsa Caeiro2, Ana Todo-Bom3, Ana Duarte4, Luiz Gazarini5. 1. Serviço de Patologia Clínica, Hospital José Joaquim Fernandes, Rua Dr. António Fernando Covas Lima, 7801-849 Beja, Portugal; Universidade de Évora, Évora, Portugal. Electronic address: elsaalmeida.1983@gmail.com. 2. Sociedade Portuguesa de Alergologia e Imunologia Clínica - SPAIC, Lisboa, Portugal; Instituto de Ciências Agrárias e Ambientais Mediterrânicas - ICAAM, Universidade de Évora, Évora, Portugal. Electronic address: elcaeiro@yahoo.com. 3. Universidade de Coimbra, Coimbra, Portugal. Electronic address: flcosta@netcabo.pt. 4. Hospital José Joaquim Fernandes, Unidade Local de Saúde do Baixo Alentejo, Beja, Portugal. Electronic address: crisduarte@sapo.pt. 5. Universidade de Évora, Évora, Portugal; Instituto de Ciências Agrárias e Ambientais Mediterrânicas - ICAAM, Universidade de Évora, Évora, Portugal. Electronic address: gazarini@uevora.pt.
Abstract
BACKGROUND: In Portugal, the pollen types most implicated in respiratory allergy are grasses, olive and parietaria. The knowledge of sensitizations to molecular allergens in children and adults can contribute to better diagnosis and treatment of this pathology. METHODS: ImmunoCAP singleplex technology was used for molecular allergens and Phadia 250® automatic equipment. g205 (Phl p1); g215 (Phl p5b); g210 (Phl p7); and g212 (Phl p12) allergen determinations were made in 45 patients with positive grass sensitization tests. RESULTS: The majority of patients are sensitized to Phl p1 (91%) and Phl p1+/Phl p5-/Phl p7-/Phl p12- was the most dominant profile (40%). In the adult group, the IgE averages for Phl p1 were approximately 10.46, while they were 8.43 for Phl p5, 0.69 for Phl p7, and 0.06 for Phl p12. In the child group, these values were higher: 22.49, 20.23, 3.89, and 0.35, respectively. For allergens Phl p1, Phl p5, and Phl p7, these differences between the child and adult population were not statistically significant (p=0.754, p=0.806 and p=0.102, respectively), but for Phl p12, a statistically significant difference (p=0.018) was observed. CONCLUSIONS: IgE antibodies Phl p1 is the most important allergic marker and sensitivities caused by Phl p12 give rise to higher IgE values in children.
BACKGROUND: In Portugal, the pollen types most implicated in respiratory allergy are grasses, olive and parietaria. The knowledge of sensitizations to molecular allergens in children and adults can contribute to better diagnosis and treatment of this pathology. METHODS: ImmunoCAP singleplex technology was used for molecular allergens and Phadia 250® automatic equipment. g205 (Phl p1); g215 (Phl p5b); g210 (Phl p7); and g212 (Phlp12) allergen determinations were made in 45 patients with positive grass sensitization tests. RESULTS: The majority of patients are sensitized to Phl p1 (91%) and Phl p1+/Phl p5-/Phl p7-/Phlp12- was the most dominant profile (40%). In the adult group, the IgE averages for Phl p1 were approximately 10.46, while they were 8.43 for Phl p5, 0.69 for Phl p7, and 0.06 for Phlp12. In the child group, these values were higher: 22.49, 20.23, 3.89, and 0.35, respectively. For allergens Phl p1, Phl p5, and Phl p7, these differences between the child and adult population were not statistically significant (p=0.754, p=0.806 and p=0.102, respectively), but for Phlp12, a statistically significant difference (p=0.018) was observed. CONCLUSIONS: IgE antibodies Phl p1 is the most important allergic marker and sensitivities caused by Phlp12 give rise to higher IgE values in children.
Authors: Galber R Araujo; Lorenz Aglas; Emília R Vaz; Yoan Machado; Sara Huber; Martin Himly; Albert Duschl; Luiz R Goulart; Fatima Ferreira Journal: Allergy Date: 2019-12-12 Impact factor: 13.146