Mingjiang Bie1,2,3,4, Jirui Wen1, Hongren Wang1, Linlin Zhou1, Lijun Yang2, Yongyue Pan2, Jun Luo1, Chao Liu5, Mingyuan Li1, Baoning Wang1. 1. West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu 610041, China. 2. Medical College of Tibet University, Lhasa 850000, China. 3. West China Fourth Hospital of Public Health, Sichuan University, Chengdu 610041, China. 4. Editorial Board of Journal of Sichuan University (Medical Science Edition), Chengdu 610041, China. 5. Hospital of Chengdu Office, People's Government of Tibet Autonomous Region, Chengdu 610041, China.
Abstract
BACKGROUND: Helicobacter pylori (H. pylori) is a Gram-negative pathogenic bacterium that causes chronic gastritis and other gastric diseases in humans. In Tibet, China, the infection of H. pylori is an important risk factor that caused gastric cancer. METHODS: To understand the characteristics of this pathogen in Tibet, five strains of H. pylori were isolated from three patients' oral cavity or stomach who had either a gastric ulcer or gastritis. We performed genome sequences of these five clinical strains on Illumina Hiseq, and 55,016-63666 SNVs/InDels were identified by comparing to the reference strain of H. pylori 26995. RESULTS: The phylogenetic analysis with multi-locus sequence typing (MLST) showed that five Tibetan strains were defined as hpEurope population and their proteins encoded by the cagA gene also presented a western type. Also, the strains that were isolated from the same patients' oral cavity and stomach exhibited homology in molecular evolution. CONCLUSIONS: This is the first study to investigate the phylogenetic population structure of the epidemic strains of H. pylori in Tibet, which may improve cognition of Tibetan strains and confirm the homology of the strains from oral cavity and stomach.
BACKGROUND: Helicobacter pylori (H. pylori) is a Gram-negative pathogenic bacterium that causes chronic gastritis and other gastric diseases in humans. In Tibet, China, the infection of H. pylori is an important risk factor that caused gastric cancer. METHODS: To understand the characteristics of this pathogen in Tibet, five strains of H. pylori were isolated from three patients' oral cavity or stomach who had either a gastric ulcer or gastritis. We performed genome sequences of these five clinical strains on Illumina Hiseq, and 55,016-63666 SNVs/InDels were identified by comparing to the reference strain of H. pylori 26995. RESULTS: The phylogenetic analysis with multi-locus sequence typing (MLST) showed that five Tibetan strains were defined as hpEurope population and their proteins encoded by the cagA gene also presented a western type. Also, the strains that were isolated from the same patients' oral cavity and stomach exhibited homology in molecular evolution. CONCLUSIONS: This is the first study to investigate the phylogenetic population structure of the epidemic strains of H. pylori in Tibet, which may improve cognition of Tibetan strains and confirm the homology of the strains from oral cavity and stomach.
Entities:
Keywords:
Helicobacter pylori (H. pylori); Tibet; multi locus sequence typing; oral strain
Authors: M Achtman; T Azuma; D E Berg; Y Ito; G Morelli; Z J Pan; S Suerbaum; S A Thompson; A van der Ende; L J van Doorn Journal: Mol Microbiol Date: 1999-05 Impact factor: 3.501
Authors: Daniel Falush; Thierry Wirth; Bodo Linz; Jonathan K Pritchard; Matthew Stephens; Mark Kidd; Martin J Blaser; David Y Graham; Sylvie Vacher; Guillermo I Perez-Perez; Yoshio Yamaoka; Francis Mégraud; Kristina Otto; Ulrike Reichard; Elena Katzowitsch; Xiaoyan Wang; Mark Achtman; Sebastian Suerbaum Journal: Science Date: 2003-03-07 Impact factor: 47.728
Authors: B W Loster; S W Majewski; M Cześnikiewicz-Guzik; W Bielanski; P Pierzchalski; S J Konturek Journal: J Physiol Pharmacol Date: 2006-09 Impact factor: 3.011
Authors: Maria G Domínguez-Bello; Maria E Pérez; Maria C Bortolini; Francisco M Salzano; Luis R Pericchi; Orlisbeth Zambrano-Guzmán; Bodo Linz Journal: PLoS One Date: 2008-10-02 Impact factor: 3.240