Gülden Ereş1, Ceren Su Akgün Demirtaş2, Ece Toptaş3, Ayça Dilara Yılmaz4, Burcu Sengüven5, Kıvanç Kamburoğlu6. 1. Department of Periodontology, Faculty of Dentistry, Ankara University, Ankara, Turkey. Electronic address: guldeneres@gmail.com. 2. Department of Periodontology, Faculty of Dentistry, Ankara University, Ankara, Turkey. Electronic address: cerensuakgun@hotmail.com. 3. Department of Periodontology, Faculty of Dentistry, Ankara University, Ankara, Turkey. Electronic address: ecerakunt@gmail.com. 4. Molecular Biology Laboratory, Faculty of Dentistry, Ankara University, Ankara, Turkey. Electronic address: aycad.yilmaz@gmail.com. 5. Department of Oral Pathology, Faculty of Dentistry, Gazi University, Ankara, Turkey. Electronic address: burcu.senguven@gmail.com. 6. Department of Dentomaxillofacial Radiology, Faculty of Dentistry, Ankara University, Ankara, Turkey. Electronic address: Dtkivo@yahoo.com.
Abstract
OBJECTIVE: The aim of this study was to examine the correlations between the levels of ghrelin and inflammatory and bone metabolism markers in rats with periodontitis. DESIGN: Thirty female Wistar rats (6 trial rats and 4 control rats in each group) were divided into pubertal, adult and postmenopausal groups. Periodontitis was induced by ligatures. On the 21 st day, blood was collected and all rats were then sacrificed. The levels of osteocalcin, osteoprotegerin, alkaline phosphatase, tumour necrosis factor alpha (TNF-α), interleukin 1 beta (IL-1β), acylated ghrelin, total ghrelin and soluble receptor activator of nuclear factor-kB ligands in the blood samples were measured using enzyme-linked immunosorbent assays. The jaws were decalcified in a Tris-EDTA solution and embedded in paraffin and 4-5 μm sections were cut for IL-β, TNF -α and ghrelin staining. RESULTS: Significantly higher serum alkaline phosphatase levels were detected in the trial rats in the pubertal group than in the control rats (p = 0.033). In the postmenopausal group, ghrelin levels positively correlated with interleukin 1 beta levels (r = 0.708, p < 0.05). Among all trial rats, the postmenopausal group exhibited significantly higher levels of acylated ghrelin than the other groups (p = 0.001). Significantly higher osteoprotegerin levels were observed in the control rats than in the trial rats in the postmenopausal group (p = 0.012). Inflammation scores were significantly higher in adult trial rats than in controls (p = 0.024); significantly higher TNF-α levels were detected in postmenopausal experimental rats than in the adult experimental group (p = 0.025). CONCLUSIONS: We concluded that total ghrelin levels in serum only correlated with IL-β levels in postmenopausal rats.
OBJECTIVE: The aim of this study was to examine the correlations between the levels of ghrelin and inflammatory and bone metabolism markers in rats with periodontitis. DESIGN: Thirty female Wistar rats (6 trial rats and 4 control rats in each group) were divided into pubertal, adult and postmenopausal groups. Periodontitis was induced by ligatures. On the 21 st day, blood was collected and all rats were then sacrificed. The levels of osteocalcin, osteoprotegerin, alkaline phosphatase, tumour necrosis factor alpha (TNF-α), interleukin 1 beta (IL-1β), acylated ghrelin, total ghrelin and soluble receptor activator of nuclear factor-kB ligands in the blood samples were measured using enzyme-linked immunosorbent assays. The jaws were decalcified in a Tris-EDTA solution and embedded in paraffin and 4-5 μm sections were cut for IL-β, TNF -α and ghrelin staining. RESULTS: Significantly higher serum alkaline phosphatase levels were detected in the trial rats in the pubertal group than in the control rats (p = 0.033). In the postmenopausal group, ghrelin levels positively correlated with interleukin 1 beta levels (r = 0.708, p < 0.05). Among all trial rats, the postmenopausal group exhibited significantly higher levels of acylated ghrelin than the other groups (p = 0.001). Significantly higher osteoprotegerin levels were observed in the control rats than in the trial rats in the postmenopausal group (p = 0.012). Inflammation scores were significantly higher in adult trial rats than in controls (p = 0.024); significantly higher TNF-α levels were detected in postmenopausal experimental rats than in the adult experimental group (p = 0.025). CONCLUSIONS: We concluded that total ghrelin levels in serum only correlated with IL-β levels in postmenopausal rats.
Authors: Milind V Masule; Sumit Rathod; Yogeeta Agrawal; Chandragouda R Patil; Kartik T Nakhate; Shreesh Ojha; Sameer N Goyal; Umesh B Mahajan Journal: Curr Res Pharmacol Drug Discov Date: 2022-06-13