| Literature DB >> 31471824 |
Aline Chiodi Borges1,2, Thalita Mayumi Castaldelli Nishime3,4, Sabrina de Moura Rovetta2, Gabriela de Morais Gouvêa Lima2, Konstantin Georgiev Kostov3, Gilmar Patrocínio Thim5, Beatriz Rossi Canuto de Menezes5, João Paulo Barros Machado6, Cristiane Yumi Koga-Ito7.
Abstract
This study aimed to evaluate the effects of cold atmospheric pressure plasma (CAPP) jet on Trichophyton rubrum growth, germination and adherence to nail. The effects of plasma jet on T. rubrum conidia germination and on mycelial growth were evaluated by in vitro assays. An ex vivo nail infection model was used to evaluate the effects on conidia adherence and infection. Biochemical analyses of nail fragments exposed or not to CAPP were performed by attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy. Plasma jet exposure for 10 and 15 min completely inhibited mycelial growth after only one exposure. Fifteen minutes of exposure could reduce conidia germination in suspension. Fungal suspensions exposed to plasma jet for 10 and 15 min were not able to infect nail specimens. These results were corroborated by ATR-FTIR analyses of nail fragments. In conclusion, single exposure to CAPP for 15 min was able to inhibit fungal growth, adherence and infection capacity. The results suggest that cold atmospheric plasma jet can be a promising alternative for the treatment of onychomycoses caused by T. rubrum.Entities:
Keywords: Antifungal agents; Cold plasma; Onychomycosis; Trichophyton rubrum
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Year: 2019 PMID： 31471824 DOI： 10.1007/s11046-019-00375-2
Source DB: PubMed Journal: Mycopathologia ISSN： 0301-486X Impact factor: 2.574