Y U Hirano1, Kentaro Suzuki1, Taisen Iguchi2, Gen Yamada3, Shinichi Miyagawa4. 1. Department of Developmental Genetics, Institute of Advanced Medicine, Wakayama Medical University, Wakayama, Japan. 2. Graduate School of Nanobioscience, Yokohama City University, Yokohama, Japan. 3. Department of Developmental Genetics, Institute of Advanced Medicine, Wakayama Medical University, Wakayama, Japan miyagawa@rs.tus.ac.jp genyama77@yahoo.co.jp. 4. Faculty of Industrial Science and Technology, Tokyo University of Science, Tokyo, Japan miyagawa@rs.tus.ac.jp genyama77@yahoo.co.jp.
Abstract
BACKGROUND/AIM: The mouse vagina exhibits stratified squamous epithelium, which is comprised of multiple cell layers. We previously showed that erbB signaling, induced by epithelial estrogen receptor 1 (ESR1), is required for the initial differentiation of the epithelium. However, the downstream effector that mediates terminal differentiation in the apical layers remains elusive. The contribution of fibroblast growth factor (FGF) to vaginal epithelial cell differentiation was investigated. MATERIALS AND METHODS: Vaginas from wild-type or epithelium-specific Esr1 conditional knockout (cKO) mice were analyzed using immunohistochemistry and quantitative real-time RT-PCR. RESULTS: Of the FGF ligands examined, Fgf22 mRNA was significantly induced following estrogen treatment. Furthermore, FGF downstream signaling, phosphorylated FRS2 and ERK1/2 were exclusively expressed in the apical layers of the vaginal epithelium. No changes in such expression were observed in the Esr1 cKO mice. CONCLUSION: FGF-ERK/MAPK pathway may be a main inducer of terminal differentiation in the mouse vaginal epithelium. Copyright
BACKGROUND/AIM: The mouse vagina exhibits stratified squamous epithelium, which is comprised of multiple cell layers. We previously showed that erbB signaling, induced by epithelial estrogen receptor 1 (ESR1), is required for the initial differentiation of the epithelium. However, the downstream effector that mediates terminal differentiation in the apical layers remains elusive. The contribution of fibroblast growth factor (FGF) to vaginal epithelial cell differentiation was investigated. MATERIALS AND METHODS: Vaginas from wild-type or epithelium-specific Esr1 conditional knockout (cKO) mice were analyzed using immunohistochemistry and quantitative real-time RT-PCR. RESULTS: Of the FGF ligands examined, Fgf22 mRNA was significantly induced following estrogen treatment. Furthermore, FGF downstream signaling, phosphorylated FRS2 and ERK1/2 were exclusively expressed in the apical layers of the vaginal epithelium. No changes in such expression were observed in the Esr1 cKO mice. CONCLUSION: FGF-ERK/MAPK pathway may be a main inducer of terminal differentiation in the mouse vaginal epithelium. Copyright
Authors: Klementina Fon Tacer; Angie L Bookout; Xunshan Ding; Hiroshi Kurosu; George B John; Lei Wang; Regina Goetz; Moosa Mohammadi; Makoto Kuro-o; David J Mangelsdorf; Steven A Kliewer Journal: Mol Endocrinol Date: 2010-07-28
Authors: Jürgen Kopp; Guang Y Wang; Peter Kulmburg; Stefan Schultze-Mosgau; Jing N Huan; Kang Ying; Harun Seyhan; Mark D Jeschke; Ulrich Kneser; Alexander D Bach; Sheng D Ge; Steven Dooley; Raymund E Horch Journal: Mol Ther Date: 2004-07 Impact factor: 11.454