Lara Schorn1, Jörg Handschel2, Julian Lommen1, Felix Paulssen VON Beck3, Rita Depprich1, Norbert Kübler1, Henrik Holtmann4. 1. Department of Oral-, Maxillo- and Plastic Facial Surgery, Heinrich-Heine-University and University Hospital Duesseldorf, Duesseldorf, Germany. 2. Klinik und Praxisgemeinschaft am Kaiserteich, Düsseldorf, Germany handschel@klinikamkaiserteich.de. 3. Department of Oral-, Maxillo- and Plastic Facial Surgery, Malteser Krankenhaus, St. Josefshospital Uerdingen, Krefeld-Uerdingen, Germany. 4. Department of Oral-, Maxillo- and Plastic Facial Surgery, Malteser Krankenhaus St. Johannes-Stift, Duisburg, Germany.
Abstract
BACKGROUND/AIM: Results of Guided Bone Regeneration (GBR) primarily depend on the membrane used. The aim of this study was to compare biocompatibility of different absorbable and non-absorbable membranes by using unrestricted somatic stem cells (USSCs) as an indicator for biocompatibility. MATERIALS AND METHODS: Five absorbable membranes (Bio-Gide®, RESODONT®, GENTA-FOIL resorb®, BioMend® and BioMend® Extend™) and one non-absorbable alternative (GORE-TEX®) were colonized with USSCs. After 24 h, 3 days and 7 days, cell proliferation, cell viability, and cytotoxicity were assessed. Moreover, cell morphology was evaluated by electron microscopy. RESULTS: Significantly higher cell proliferation and cell viability rates were observed in Bio-Gide® and RESODONT® membranes. Cell toxicity was highest on GENTA-FOIL resorb® membranes. The electron microscopical assessment showed a better cell attachment on porous surfaced membranes. CONCLUSION: This study shows that USSCs can be used for assessments of biocompatibility, and that absorbable membranes with collagenous composition and porous structure tend to positively impact biocompatibility and enhance cell proliferation. Copyright
BACKGROUND/AIM: Results of Guided Bone Regeneration (GBR) primarily depend on the membrane used. The aim of this study was to compare biocompatibility of different absorbable and non-absorbable membranes by using unrestricted somatic stem cells (USSCs) as an indicator for biocompatibility. MATERIALS AND METHODS: Five absorbable membranes (Bio-Gide®, RESODONT®, GENTA-FOIL resorb®, BioMend® and BioMend® Extend™) and one non-absorbable alternative (GORE-TEX®) were colonized with USSCs. After 24 h, 3 days and 7 days, cell proliferation, cell viability, and cytotoxicity were assessed. Moreover, cell morphology was evaluated by electron microscopy. RESULTS: Significantly higher cell proliferation and cell viability rates were observed in Bio-Gide® and RESODONT® membranes. Cell toxicity was highest on GENTA-FOIL resorb® membranes. The electron microscopical assessment showed a better cell attachment on porous surfaced membranes. CONCLUSION: This study shows that USSCs can be used for assessments of biocompatibility, and that absorbable membranes with collagenous composition and porous structure tend to positively impact biocompatibility and enhance cell proliferation. Copyright
Authors: Lara Schorn; Christoph Sproll; Michelle Ommerborn; Christian Naujoks; Norbert R Kübler; Rita Depprich Journal: Head Face Med Date: 2017-06-07 Impact factor: 2.151