| Literature DB >> 31466820 |
Iva Pernicova1, Dan Kucera1, Jana Nebesarova2, Michal Kalina1, Ivana Novackova1, Martin Koller3, Stanislav Obruca4.
Abstract
The aim of this work was to study the potential of selected Halomonas species for conversion of waste frying oil into polyhydroxyalkanoates (PHA). In total nine Halomonas strains were experimentally screened for their capability of PHA production. Among them, Halomonas neptunia and Halomonas hydrothermalis were identified as potent PHA producers. Initial concentration of NaCl was identified as parameter influencing PHA yields as well as molecular weight of the polymer. In addition, H. hydrothermalis was capable of biosynthesis of a copolymer of 3-hydroxybutyrate and 3-hydroxyvalerate P(3HB-co-3HV). When valerate was utilized as a precursor, the 3HV fraction in the copolymer reached high values of 50.15 mol.%. PHA production on lipid substrates by Halomonas has not been reported so far. Bearing in mind all the positive aspects of employing extremophiles in industrial biotechnology, H. hydrothermalis seems to be a very interesting halophilic strain for production of PHA using lipid substrates.Entities:
Keywords: Halomonas spp.; Halophiles; Lipids; Polyhydroxyalkanoates; Waste frying oil
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Year: 2019 PMID: 31466820 DOI: 10.1016/j.biortech.2019.122028
Source DB: PubMed Journal: Bioresour Technol ISSN: 0960-8524 Impact factor: 9.642