| Literature DB >> 31465820 |
Lei Chen1, Kang Li2, Qian Liu3, José L Quiles4, Rosanna Filosa5, Mohammad Amjad Kamal6, Fang Wang7, Guoyin Kai8, Xiaobo Zou9, Hui Teng10, Jianbo Xiao11.
Abstract
The aim of the present study was to explore the protective effects of raspberry and its bioactive compound cyanidin 3-O-glucoside against H2O2-induced oxidative stress in HepG2 cells. We established a model of oxidative stress in HepG2 cells induced by H2O2 and examined the protein expression of Keap1/Nrf2. The antioxidant activity of raspberry extract was carried out measuring the level of reactive oxygen species (ROS), and the changes of phase II detoxification elements such as GSH level and CAT activity. Also the expression of proteins related to the Keap1/Nrf2 signaling was tested. The results revealed that raspberry extract significantly reduced the ROS levels in oxidative injured cells, increased GSH content and CAT activity, and activated the expression of proteins Keap1, Nrf2, HO-1, NQO1, and γ-GCS. These results taken together indicated that raspberry treatment could ameliorate H2O2-induced oxidative stress in HepG2 cells via Keap1/Nrf2 pathway.Entities:
Keywords: Cyanidin 3-O-Glucoside; HepG2 cells; Keap1/Nrf2 pathway; Oxidative damage; Raspberry
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Year: 2019 PMID： 31465820 DOI： 10.1016/j.fct.2019.110781
Source DB: PubMed Journal: Food Chem Toxicol ISSN： 0278-6915 Impact factor: 6.023