In vitro fermentation of Pennisetum clandestinum Hochst. Ex Chiov increased methane production with ruminal fluid adapted to crude glycerol.

The aim of this trial was to determine the effect of ruminal fluid adapted to crude glycerol supplementation on in vitro methane and fermentation parameters of the forage Pennisetum clandestinum Hochst. Ex Chiov (Kikuyu forage) of 35 (D35) and 45 days (D45) of regrowth. Four non-lactating cannulated Holstein cows with 600 ± 33 kg LW, under a randomized Latin square design (4 animals × 4 periods), were used to obtain ruminal fluid after adaptation for 13 days within each period to supplementation in the rumen with crude glycerol (GL): 0 (GL0), 500 (GL1), 1000 (GL2), and 1500 g/cow/day (GL3). All the variables were analyzed under a 4 × 2 split-plot arrangement, where the ruminal fluid adaptation to supplementation with GL and its interaction with the incubated diets (GL× D) were analyzed. Ruminal pH values and in vitro apparent degradability of the dry matter (DMd) and organic matter (OMd) were not affected, with a decrease in the concentrations of NH3-N and acetate by the ruminal fluid adapted to crude glycerol. The concentrations of propionate and butyrate increased, without affecting the total concentration of VFA in the ruminal fluid. The in vitro methane production increased with forage D35 and GL1, GL2, GL3. The in vitro methane production with the D45 forage increased with the ruminal fluid adapted to GL2 and GL3. The ruminal fluid adapted to crude glycerol supplementation increased in vitro methane production when incubating the forage Kikuyu.