Zhenyuan Yu1,2,3,4,5, Jinling Liao2,4,5, Yang Chen1,2,3,4,5, Chunlin Zou6,7, Haiying Zhang2,4,5, Jiwen Cheng1,2,3,4,5, Deyun Liu1,3, Tianyu Li1,3, Qingyun Zhang1,2,4,5,8, Jiaping Li9,10,11, Xiaobo Yang2,4,5, Yu Ye1,2,4,5,12, Zhiguang Huang2,4,5, Xinyang Long2,4,5, Rirong Yang13,4,5,14, Zengnan Mo15,2,3,4,5. 1. Institute of Urology and Nephrology. 2. Center for Genomic and Personalized Medicine. 3. Departments of Urology and. 4. Guangxi Collaborative Innovation Center for Genomic and Personalized Medicine, Nanning, China. 5. Guangxi Key Laboratory for Genomic and Personalized Medicine, Guangxi Key Laboratory of Colleges and Universities, Nanning, China. 6. Key Laboratory of Longevity and Ageing-related Diseases, Ministry of Education, Nanning, China. 7. Center for Translational Medicine, Guangxi Medical University, Nanning, China. 8. Department of Urology, Affiliated Tumor Hospital of Guangxi Medical University, Nanning, China. 9. Cardiology, The First Affiliated Hospital of Guangxi Medical University, Nanning, China. 10. Guangxi Key Laboratory of Precision Medicine in Cardio-cerebrovascular Diseases Control and Prevention, Nanning, China. 11. Guangxi Clinical Research Center for Cardio-cerebrovascular Diseases, Nanning, China; and. 12. Scientific Research Department, The Second Affiliated Hospital of Guangxi Medical University, Nanning, China. 13. Center for Genomic and Personalized Medicine, zengnanmo@hotmail.com 520yrr@163.com. 14. Department of Immunology, School of Preclinical Medicine, Guangxi Medical University, Nanning, China. 15. Institute of Urology and Nephrology, zengnanmo@hotmail.com 520yrr@163.com.
Abstract
BACKGROUND: Having a comprehensive map of the cellular anatomy of the normal human bladder is vital to understanding the cellular origins of benign bladder disease and bladder cancer. METHODS: We used single-cell RNA sequencing (scRNA-seq) of 12,423 cells from healthy human bladder tissue samples taken from patients with bladder cancer and 12,884 cells from mouse bladders to classify bladder cell types and their underlying functions. RESULTS: We created a single-cell transcriptomic map of human and mouse bladders, including 16 clusters of human bladder cells and 15 clusters of mouse bladder cells. The homology and heterogeneity of human and mouse bladder cell types were compared and both conservative and heterogeneous aspects of human and mouse bladder evolution were identified. We also discovered two novel types of human bladder cells. One type is ADRA2A + and HRH2 + interstitial cells which may be associated with nerve conduction and allergic reactions. The other type is TNNT1 + epithelial cells that may be involved with bladder emptying. We verify these TNNT1 + epithelial cells also occur in rat and mouse bladders. CONCLUSIONS: This transcriptomic map provides a resource for studying bladder cell types, specific cell markers, signaling receptors, and genes that will help us to learn more about the relationship between bladder cell types and diseases.
BACKGROUND: Having a comprehensive map of the cellular anatomy of the normal human bladder is vital to understanding the cellular origins of benign bladder disease and bladder cancer. METHODS: We used single-cell RNA sequencing (scRNA-seq) of 12,423 cells from healthy human bladder tissue samples taken from patients with bladder cancer and 12,884 cells from mouse bladders to classify bladder cell types and their underlying functions. RESULTS: We created a single-cell transcriptomic map of human and mouse bladders, including 16 clusters of human bladder cells and 15 clusters of mouse bladder cells. The homology and heterogeneity of human and mouse bladder cell types were compared and both conservative and heterogeneous aspects of human and mouse bladder evolution were identified. We also discovered two novel types of human bladder cells. One type is ADRA2A + and HRH2 + interstitial cells which may be associated with nerve conduction and allergic reactions. The other type is TNNT1 + epithelial cells that may be involved with bladder emptying. We verify these TNNT1 + epithelial cells also occur in rat and mouse bladders. CONCLUSIONS: This transcriptomic map provides a resource for studying bladder cell types, specific cell markers, signaling receptors, and genes that will help us to learn more about the relationship between bladder cell types and diseases.
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