Literature DB >> 3145906

Characterization of signal-sequence-coding regions selected from the Bacillus subtilis chromosome.

H Smith1, A de Jong, S Bron, G Venema.   

Abstract

Signal-sequence-coding regions for protein export were selected from chromosomal Bacillus subtilis DNA. The number of different signals obtained was higher than expected on the basis of known exported proteins in B. subtilis. Most of the selected regions showed the characteristics of typical signal sequences, including a basic N-terminal region followed by a hydrophobic core and a potential signal-peptidase cleavage site. The signal-coding regions were functionally interchangeable between the B. licheniformis alpha-amylase and Escherichia coli TEM beta-lactamase genes. In addition to the signal-sequence-coding regions, the nature of the host cells, and the mature parts of the reporter proteins contributed to the amounts of protein secreted.

Entities:  

Mesh：

Substances：

Year:  1988        PMID： 3145906     DOI： 10.1016/0378-1119(88)90207-7

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  16 in total

1.  A rapid protein folding assay for the bacterial periplasm.

Authors:  Thomas J Mansell; Stephen W Linderman; Adam C Fisher; Matthew P DeLisa
Journal:  Protein Sci       Date:  2010-05       Impact factor: 6.725

2.  Infection-derived Enterococcus faecalis strains are enriched in esp, a gene encoding a novel surface protein.

Authors:  V Shankar; A S Baghdayan; M M Huycke; G Lindahl; M S Gilmore
Journal:  Infect Immun       Date:  1999-01       Impact factor: 3.441

Review 3.  Protein secretion in Bacillus species.

Authors:  M Simonen; I Palva
Journal:  Microbiol Rev       Date:  1993-03

4.  Evaluation of bottlenecks in the late stages of protein secretion in Bacillus subtilis.

Authors:  A Bolhuis; H Tjalsma; H E Smith; A de Jong; R Meima; G Venema; S Bron; J M van Dijl
Journal:  Appl Environ Microbiol       Date:  1999-07       Impact factor: 4.792

5.  Combinatorial mutagenesis and selection of improved signal sequences and their application for high-level production of translocated heterologous proteins in Escherichia coli.

Authors:  Tonje Marita Bjerkan Heggeset; Veronika Kucharova; Ingemar Naerdal; Svein Valla; Håvard Sletta; Trond Erling Ellingsen; Trygve Brautaset
Journal:  Appl Environ Microbiol       Date:  2012-11-09       Impact factor: 4.792

6.  Signal peptidase I overproduction results in increased efficiencies of export and maturation of hybrid secretory proteins in Escherichia coli.

Authors:  J M van Dijl; A de Jong; H Smith; S Bron; G Venema
Journal:  Mol Gen Genet       Date:  1991-05

7.  Secretion of TEM beta-lactamase with signal sequences isolated from the chromosome of Lactococcus lactis subsp. lactis.

Authors:  M Sibakov; T Koivula; A von Wright; I Palva
Journal:  Appl Environ Microbiol       Date:  1991-02       Impact factor: 4.792

8.  Identification and characterization of pbpC, the gene encoding Bacillus subtilis penicillin-binding protein 3.

Authors:  T Murray; D L Popham; P Setlow
Journal:  J Bacteriol       Date:  1996-10       Impact factor: 3.490

9.  A gene (sleB) encoding a spore cortex-lytic enzyme from Bacillus subtilis and response of the enzyme to L-alanine-mediated germination.

Authors:  R Moriyama; A Hattori; S Miyata; S Kudoh; S Makino
Journal:  J Bacteriol       Date:  1996-10       Impact factor: 3.490

10.  Protein export elements from Lactococcus lactis.

Authors:  G Perez-Martinez; J Kok; G Venema; J M van Dijl; H Smith; S Bron
Journal:  Mol Gen Genet       Date:  1992-09
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.