Zhi-Wen Luo1,2, Han-Tsing Wang3,4, Ning Wang1,2, Wei-Wei Sheng1,2, Ming Jin1, Ye Lu1, Yi-Jiang Bai1,2, Su-Qi Zou3,4, Yu-Lian Pang1, Hong Xu3,4, Xu Zhang1,4. 1. Affiliated Eye Hospital of Nanchang University; Jiangxi Research Institute of Ophthalmology & Visual Science, Nanchang 330006, Jiangxi Province, China. 2. Queen Mary School of Nanchang University, Nanchang 330031, Jiangxi Province, China. 3. Institute of Life Science, Nanchang University, Nanchang 330031, Jiangxi Province, China. 4. Jiangxi Provincial Collaborative Innovation Center for Cardiovascular, Digestive and Neuropsychiatric Diseases, Nanchang 330031, Jiangxi Province, China.
Abstract
AIM: To establish a model of retinal neurodegeneration induced by N-Methyl-D-aspartic acid (NMDA) in adult zebrafish. METHODS: We compared the effects of three different NMDA delivery methods on retinal neurodegeneration in adult zebrafish: immersion (I.M.), intravitreal injection (I.V.), and intraperitoneal injection (I.P.), and examined retinal pathology and degeneration by hematoxylin and eosin and TUNEL staining in the treated zebrafish. Effects of the NMDA receptor antagonist MK-801 and the natural product resveratrol on NMDA-induced retinal neurodegeneration were also assessed. RESULTS: The thickened inner retina was seen in histology with 100 µmol/L NMDA by I.M. administration. Significant apoptosis in the retinal ganglion cell layer and retinal thickness reduction occurred in 0.5 mol/L NMDA I.P. administration group.Seizure-like behavioral changes, but no retinal histological alteration occurred in 16 mg/kg NMDA I.P. administration group. Resveratrol and MK-801 prevented NMDA-induced retinal neurodegeneration in the zebrafish. CONCLUSION: Among the three drug administration methods, I.V. injection of NMDA is the most suitable for establishment of an acute retinal damage model in zebrafish. I.M. with NMDA is likely the best for use as a chronic retinal damage model. I.P. treatment with NMDA causes brain damage. Resveratrol and MK801 may be a clinically valuable treatment for retinal neurodegeneration.
AIM: To establish a model of retinal neurodegeneration induced by N-Methyl-D-aspartic acid (NMDA) in adult zebrafish. METHODS: We compared the effects of three different NMDA delivery methods on retinal neurodegeneration in adult zebrafish: immersion (I.M.), intravitreal injection (I.V.), and intraperitoneal injection (I.P.), and examined retinal pathology and degeneration by hematoxylin and eosin and TUNEL staining in the treated zebrafish. Effects of the NMDA receptor antagonist MK-801 and the natural product resveratrol on NMDA-induced retinal neurodegeneration were also assessed. RESULTS: The thickened inner retina was seen in histology with 100 µmol/L NMDA by I.M. administration. Significant apoptosis in the retinal ganglion cell layer and retinal thickness reduction occurred in 0.5 mol/L NMDA I.P. administration group.Seizure-like behavioral changes, but no retinal histological alteration occurred in 16 mg/kg NMDA I.P. administration group. Resveratrol and MK-801 prevented NMDA-induced retinal neurodegeneration in the zebrafish. CONCLUSION: Among the three drug administration methods, I.V. injection of NMDA is the most suitable for establishment of an acute retinal damage model in zebrafish. I.M. with NMDA is likely the best for use as a chronic retinal damage model. I.P. treatment with NMDA causes brain damage. Resveratrol and MK801 may be a clinically valuable treatment for retinal neurodegeneration.
Authors: Brooke Turkalj; Danielle Quallich; Denise A Bessert; Ashley C Kramer; Tiffany A Cook; Ryan Thummel Journal: Exp Eye Res Date: 2021-05-21 Impact factor: 3.770
Authors: Salvatore L Stella; Jasmine S Geathers; Sarah R Weber; Michael A Grillo; Alistair J Barber; Jeffrey M Sundstrom; Stephanie L Grillo Journal: Cells Date: 2021-03-12 Impact factor: 6.600