Li-Xin Wang1, Rui-Xia Ma2,3, Ling-Ling Di4, Xian-Bing Peng1, Zhao-Peng Kang5, Sen Zhong6. 1. Department of Otolaryngology, Renmin Hospital, Hubei University of Medicine, Shiyan, 442000, Hubei, People's Republic of China. 2. Department of Otolaryngology Head and Neck Surgery, The First People's Hospital of Yinchuan, Yinchuan, China. 3. Department of Otolaryngology Head and Neck Surgery, The Second Clinical College of Ningxia Medical University, Yinchuan, China. 4. Department of Otolaryngology Head and Neck Surgery, Hospital Affiliated to Ningxia Medical University, Yinchuan, China. 5. Department of Andrology, Renmin Hospital, Hubei University of Medicine, No. 39 Middle Chaoyang Road, Maojian District, Shiyan, 442000, Hubei, People's Republic of China. 407416410@qq.com. 6. Admin Office, Renmin Hospital, Hubei University of Medicine, No. 39 Middle Chaoyang Road, Maojian District, Shiyan, 442000, Hubei, People's Republic of China. 1043275363@qq.com.
Abstract
OBJECTIVE: We investigated the correlation between the expression of IL-17A in nasopharyngeal carcinoma tissues and cells and the occurrence and development of NPC was also investigated. METHODS: Forty-five NPC biopsy specimens from January 2014 to January 2016 were selected. Forty-five NPC tissue specimens and 45 chronic nasopharyngitis tissue samples were detected by immunohistochemistry. Statistical methods were used to analyze the correlation between IL-17A expression and the clinicopathological variables of NPC. The NPC patients were followed up. The levels of IL-17A mRNA in 40 NPC tissue specimens and 45 chronic nasopharyngitis tissue samples were detected by real-time PCR. IL-17A expression in 15 NPC tissue specimens and chronic nasopharyngitis tissue samples was further detected by Western blotting assays. RESULTS: IL-17A expression in NPC tissues was significantly higher than that of chronic nasopharyngitis tissues (P < 0.05). IL-17A was expressed in the nucleus and cytoplasm of both NPC tissues and chronic nasopharyngitis tissues. Stage III + IV NPC, tumor volume ≥ 50 mm, and hepatic envelope invasion and cervical lymph node metastasis were associated with significantly higher IL-17A levels versus stage I + II NPC, tumor size < 50 mm, no membrane invasion and lack of cervical lymph node metastasis (P < 0.05). IL-17A was statistically associated with tissue differentiation, serum EBV-lgA levels, and EBV infection. IL-17A-positive patients had significantly longer median survival versus IL-17A-negative patients (21.0 vs. 13.0 months, log-rank test: P < 0.05). Furthermore, 65% (26/40) of NPC tissue samples had significantly higher IL-17A mRNA levels than chronic nasopharyngitis (P < 0.05). IL-17A expression was significantly higher in NPC ≥ 50 mm, stage III + IV NPC and NPC with cervical lymph node invasion than its corresponding chronic nasopharyngitis tissue. CONCLUSION: IL-17A may be involved in the regulation of various malignant biological behaviors of NPC, which is closely related to the occurrence and development of NPC.
OBJECTIVE: We investigated the correlation between the expression of IL-17A in nasopharyngeal carcinoma tissues and cells and the occurrence and development of NPC was also investigated. METHODS: Forty-five NPC biopsy specimens from January 2014 to January 2016 were selected. Forty-five NPC tissue specimens and 45 chronic nasopharyngitis tissue samples were detected by immunohistochemistry. Statistical methods were used to analyze the correlation between IL-17A expression and the clinicopathological variables of NPC. The NPC patients were followed up. The levels of IL-17A mRNA in 40 NPC tissue specimens and 45 chronic nasopharyngitis tissue samples were detected by real-time PCR. IL-17A expression in 15 NPC tissue specimens and chronic nasopharyngitis tissue samples was further detected by Western blotting assays. RESULTS:IL-17A expression in NPC tissues was significantly higher than that of chronic nasopharyngitis tissues (P < 0.05). IL-17A was expressed in the nucleus and cytoplasm of both NPC tissues and chronic nasopharyngitis tissues. Stage III + IV NPC, tumor volume ≥ 50 mm, and hepatic envelope invasion and cervical lymph node metastasis were associated with significantly higher IL-17A levels versus stage I + II NPC, tumor size < 50 mm, no membrane invasion and lack of cervical lymph node metastasis (P < 0.05). IL-17A was statistically associated with tissue differentiation, serum EBV-lgA levels, and EBV infection. IL-17A-positive patients had significantly longer median survival versus IL-17A-negative patients (21.0 vs. 13.0 months, log-rank test: P < 0.05). Furthermore, 65% (26/40) of NPC tissue samples had significantly higher IL-17A mRNA levels than chronic nasopharyngitis (P < 0.05). IL-17A expression was significantly higher in NPC ≥ 50 mm, stage III + IV NPC and NPC with cervical lymph node invasion than its corresponding chronic nasopharyngitis tissue. CONCLUSION:IL-17A may be involved in the regulation of various malignant biological behaviors of NPC, which is closely related to the occurrence and development of NPC.
Authors: Marie-Laure Michel; Daniella Mendes-da-Cruz; Alexandre Castro Keller; Matthias Lochner; Elke Schneider; Michel Dy; Gérard Eberl; Maria C Leite-de-Moraes Journal: Proc Natl Acad Sci U S A Date: 2008-12-04 Impact factor: 11.205
Authors: Sofia Buonocore; Philip P Ahern; Holm H Uhlig; Ivaylo I Ivanov; Dan R Littman; Kevin J Maloy; Fiona Powrie Journal: Nature Date: 2010-04-29 Impact factor: 49.962