| Literature DB >> 31456007 |
Tamara Jiménez-Gόngora1,2, Huang Tan1,2, Rosa Lozano-Durán3.
Abstract
KEY MESSAGE: Agrobacterium-mediated transient overexpression of E2Fb triggers new cell divisions in pavement cells of Nicotiana benthamiana leaves. Transient transformation in Nicotiana benthamiana enables the study of multiple biological processes in a simple and fast manner. Here, we describe that, upon A. tumefaciens-mediated transient overexpression of the cell cycle regulator E2Fb from either Arabidopsis thaliana or N. benthamiana, cell divisions occur in epidermal pavement cells in N. benthamiana leaves, following a sequence of events that encompasses the nucleus taking a central position and being surrounded by chloroplasts, nuclear division, and formation of a new wall that divides the initial cell in two. Our results indicate that transient expression in N. benthamiana can be used to study cell division in plants, from DNA replication to cell wall formation, in a simple, controlled, and rapid manner.Entities:
Keywords: Cell division; E2Fb; Nicotiana bentamiana; Pavement cell; Transient expression
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Year: 2019 PMID： 31456007 DOI： 10.1007/s00299-019-02457-3
Source DB: PubMed Journal: Plant Cell Rep ISSN： 0721-7714 Impact factor: 4.570