| Literature DB >> 31452159 |
Dawn M Roellig1, Lihua Xiao2,3.
Abstract
Cryptosporidium genotyping has made significant contributions to the species structure and population genetics of Cryptosporidium spp. In addition, the standardized method has shown utility in epidemiologic investigations identifying case linkages and contamination sources that could not be determined with traditional epidemiologic tools. The standardized method for Cryptosporidium genotyping from stool specimens described here comprises multiple individual protocols to amplify and sequence regions of the SSU rRNA and gp60 genes of Cryptosporidium.Keywords: DNA sequencing; Genotyping; Molecular epidemiology; PCR; PCR-RFLP; Subtyping
Year: 2020 PMID： 31452159 DOI： 10.1007/978-1-4939-9748-0_7
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745