Literature DB >> 31452155

Detection of Cryptosporidium Recovered from Large-Volume Water Samples Using Dead-End Ultrafiltration.

Amy M Kahler1, Vincent R Hill2.   

Abstract

The procedure described here provides instructions for detection of Cryptosporidium recovered from large-volume water samples. Water samples are collected by dead-end ultrafiltration in the field and ultrafilters are processed in a laboratory. Microbes recovered from the filters are further concentrated and subjected to Cryptosporidium isolation or nucleic acid extraction methods for the detection of Cryptosporidium oocysts or Cryptosporidium DNA.

Entities:  

Keywords:  Cryptosporidium; Dead-end ultrafiltration; IMS; Microscopy; Real-time PCR; Water

Mesh：

Substances：

Year:  2020        PMID： 31452155      PMCID： PMC6953165          DOI： 10.1007/978-1-4939-9748-0_3

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  5 in total

1.  Ultrafiltration improves ELISA and Endopep MS analysis of botulinum neurotoxin type A in drinking water.

Authors:  Brian H Raphael; Matthew Lautenschlager; Amy Kahler; Suresh Pai; Bryan A Parks; Suzanne R Kalb; Susan E Maslanka; Sanjiv Shah; Matthew Magnuson; Vincent R Hill
Journal:  J Microbiol Methods       Date:  2012-06-05       Impact factor: 2.363

2.  Dead-end hollow-fiber ultrafiltration for recovery of diverse microbes from water.

Authors:  Carmela M Smith; Vincent R Hill
Journal:  Appl Environ Microbiol       Date:  2009-06-26       Impact factor: 4.792

3.  Recovery of diverse microbes in high turbidity surface water samples using dead-end ultrafiltration.

Authors:  Bonnie Mull; Vincent R Hill
Journal:  J Microbiol Methods       Date:  2012-10-12       Impact factor: 2.363

4.  Detection and differentiation of Cryptosporidium hominis and Cryptosporidium parvum by dual TaqMan assays.

Authors:  N Jothikumar; A J da Silva; I Moura; Y Qvarnstrom; V R Hill
Journal:  J Med Microbiol       Date:  2008-09       Impact factor: 2.472

5.  Development of a nucleic Acid extraction procedure for simultaneous recovery of DNA and RNA from diverse microbes in water.

Authors:  Vincent R Hill; Jothikumar Narayanan; Rachel R Gallen; Karen L Ferdinand; Theresa Cromeans; Jan Vinjé
Journal:  Pathogens       Date:  2015-05-26
  5 in total

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