Ultrasonic energy-assisted in-situ synthesis of Ru0/PANI/g-C3N4 nanocomposite: Application for picomolar-level electrochemical detection of endocrine disruptor (Bisphenol-A) in humans and animals.

Bisphenol A (BPA) is an endocrine-disrupting chemical which resembles structurally the hormone estrogen. Even a trace amount of BPA can bind estrogen receptors resulting in the inducement of reproductive disorders, cancers and problems related to sexual growth such as manliness in female and womanliness in male. So the determination of BPA in human and animal bodies is very essential. For this purpose, a new nanocomposite composed of ruthenium nanoparticles, polyaniline and graphitic carbon nitride (Ru0/PANI/g-C3N4) has been synthesized ultrasonically (40 ± 3 kHz, 200 W). A modification on glassy carbon electrode (GCE) with the nanocomposite detects BPA in human and animal urine samples with wide linear range (0.01-1.1 µM) and the limit of detection is pico molar-level. The synthesized nanocomposite was characterized by Ultraviolet-Visible and Fourier Transform-Infra Red spectroscopies, thermo gravimetric analysis, transmission electron microscopy, X-ray diffraction study, energy dispersive X-ray analysis, and elemental mapping analysis. This sensing system is selective, stable and reusable, by which the detection of BPA in various physiological fluids is very much possible.