Ali Parlar1, Seyfullah Oktay Arslan2. 1. Pharmacology Department, Medical Faculty, Adiyaman University, Adiyaman, Turkey. 2. Pharmacology Department, Medical Faculty, Ankara Yildirim Beyazit University, Ankara, Turkey. Electronic address: soarslan@gmail.com.
Abstract
BACKGROUND: The aim of the present study was to investigate the effect of resveratrol on cytokine levels and oxidative stress in intestinal ischemia/reperfusion (I/R) injuries. METHODS: To induce intestinal I/R, the superior mesenteric artery was occluded for 30 min and then reperfused for 150 min or 24 h. The therapeutic effects of resveratrol on the damage from intestinal I/R were investigated using an isolated organ bath, along with oxidant/antioxidant and inflammatory factors such as glutathione (GSH), malondialdehyde (MDA), myeloperoxidase (MPO), interleukin-(IL)-1β, and tumor necrosis factor alpha (TNF-α). RESULTS: I/R control animals demonstrated severe deterioration of smooth muscle motor function as a significant decrease in potassium chloride- and acetylcholine-induced a contractile responses; high oxidative stress as an increase in lipid peroxidation and a decrease in GSH level; and an increase of MPO, IL-1β, and TNF-α activity. Pretreatment of animals with resveratrol restored intestinal dysfunction; reduced elevated MDA, MPO, IL-1β, and TNF-α levels; and reversed the depleted intestine GSH levels after both 150 min and 24 h reperfusion periods. CONCLUSIONS: The results indicated that resveratrol can reverse the effects of disrupted smooth muscle contractility, probably because of its antioxidant and anti-inflammatory effects on MPO, IL-1β, and TNF-α activities.
BACKGROUND: The aim of the present study was to investigate the effect of resveratrol on cytokine levels and oxidative stress in intestinal ischemia/reperfusion (I/R) injuries. METHODS: To induce intestinal I/R, the superior mesenteric artery was occluded for 30 min and then reperfused for 150 min or 24 h. The therapeutic effects of resveratrol on the damage from intestinal I/R were investigated using an isolated organ bath, along with oxidant/antioxidant and inflammatory factors such as glutathione (GSH), malondialdehyde (MDA), myeloperoxidase (MPO), interleukin-(IL)-1β, and tumor necrosis factor alpha (TNF-α). RESULTS: I/R control animals demonstrated severe deterioration of smooth muscle motor function as a significant decrease in potassium chloride- and acetylcholine-induced a contractile responses; high oxidative stress as an increase in lipid peroxidation and a decrease in GSH level; and an increase of MPO, IL-1β, and TNF-α activity. Pretreatment of animals with resveratrol restored intestinal dysfunction; reduced elevated MDA, MPO, IL-1β, and TNF-α levels; and reversed the depleted intestine GSH levels after both 150 min and 24 h reperfusion periods. CONCLUSIONS: The results indicated that resveratrol can reverse the effects of disrupted smooth muscle contractility, probably because of its antioxidant and anti-inflammatory effects on MPO, IL-1β, and TNF-α activities.
Authors: Beata Modzelewska; Krzysztof Drygalski; Hady Razak Hady; Aleksandra Kiełczewska; Andrzej Chomentowski; Krzysztof Koryciński; Paulina Głuszyńska; Tomasz Kleszczewski Journal: Front Pharmacol Date: 2022-01-25 Impact factor: 5.810