Literature DB >> 3144971

Endogenous ADP-ribosylation in human platelets.

L Molina y Vedia1, R D Nolan, E G Lapetina.   

Abstract

We have studied ADP-ribosyltransferase activity in platelet cytosol and electropermeabilized platelets. Cytosolic activity causes ADP-ribosylation or of a 37 kDa protein that is activated by increasing the concentration of potassium phosphate. ADP-ribosylation is inhibited by thiol reagents, an effect partially reversed by cholera toxin. In electropermeabilized platelets incubated with [alpha-32P]NAD, the 37 kDa protein is also ADP-ribosylated as are other proteins and albumin. Under these conditions, ADP-ribosylation is partially inhibited by nicotinamide. This experimental design could be used to determine the effect of cell agonists on endogenous ADP-ribosylation of proteins.

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Year:  1988        PMID： 3144971     DOI： 10.1016/s0006-291x(88)81019-2

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  4 in total

1.  The effect of iloprost on the ADP-ribosylation of Gs alpha (the alpha-subunit of Gs).

Authors:  L Molina y Vedia; R D Nolan; E G Lapetina
Journal:  Biochem J       Date:  1989-08-01       Impact factor: 3.857

2.  Failure of [32P]ADP-ribosylation by pertussis toxin to determine Gi alpha content in membranes from various human tissues. Improved radioimmunological quantification using the 125I-labelled C-terminal decapeptide of retinal transducin.

Authors:  M Böhm; K Larisch; E Erdmann; M Camps; K Jakobs; P Gierschik
Journal:  Biochem J       Date:  1991-07-01       Impact factor: 3.857

3.  Agonist-induced ADP-ribosylation of a cytosolic protein in human platelets.

Authors:  B Brüne; L Molina y Vedia; E G Lapetina
Journal:  Proc Natl Acad Sci U S A       Date:  1990-05       Impact factor: 11.205

4.  Epoxyeicosatrienoic acid stimulates ADP-ribosylation of a 52 kDa protein in rat liver cytosol.

Authors:  K Seki; A Hirai; M Noda; Y Tamura; I Kato; S Yoshida
Journal:  Biochem J       Date:  1992-01-01       Impact factor: 3.857
  4 in total

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