Literature DB >> 31449258

Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells.

Aditi Bapat1, Nakia Keita1, Shalini Sharma2.   

Abstract

Ex vivo differentiation of human hematopoietic stem cells is a widely used model for studying hematopoiesis. The protocol described here is for cytokine induced differentiation of CD34+ hematopoietic stem and progenitor cells to the four myeloid lineage cells. CD34+ cells are isolated from human umbilical cord blood and co-cultured with MS-5 stromal cells in the presence of cytokines. Immunophenotypic characterization of the stem and progenitor cells, and the differentiated myeloid lineage cells are described. Using this protocol, CD34+ cells may be incubated with small molecules or transduced with lentiviruses to express myeloid disease mutations to investigate their impact on myeloid differentiation.

Entities:  

Mesh：

Substances：

Year:  2019        PMID： 31449258      PMCID： PMC7046082          DOI： 10.3791/59836

Source DB:  PubMed          Journal:  J Vis Exp        ISSN： 1940-087X            Impact factor:   1.355


  28 in total

1.  Inflammation-Induced Emergency Megakaryopoiesis Driven by Hematopoietic Stem Cell-like Megakaryocyte Progenitors.

Authors:  Simon Haas; Jenny Hansson; Daniel Klimmeck; Dirk Loeffler; Lars Velten; Hannah Uckelmann; Stephan Wurzer; Áine M Prendergast; Alexandra Schnell; Klaus Hexel; Rachel Santarella-Mellwig; Sandra Blaszkiewicz; Andrea Kuck; Hartmut Geiger; Michael D Milsom; Lars M Steinmetz; Timm Schroeder; Andreas Trumpp; Jeroen Krijgsveld; Marieke A G Essers
Journal:  Cell Stem Cell       Date:  2015-08-20       Impact factor: 24.633

2.  The generation of human innate lymphoid cells is influenced by the source of hematopoietic stem cells and by the use of G-CSF.

Authors:  Francesca Moretta; Francesca Petronelli; Barbarella Lucarelli; Angela Pitisci; Alice Bertaina; Franco Locatelli; Maria Cristina Mingari; Lorenzo Moretta; Elisa Montaldo
Journal:  Eur J Immunol       Date:  2016-02-19       Impact factor: 5.532

3.  Platelet-biased stem cells reside at the apex of the haematopoietic stem-cell hierarchy.

Authors:  Alejandra Sanjuan-Pla; Iain C Macaulay; Christina T Jensen; Petter S Woll; Tiago C Luis; Adam Mead; Susan Moore; Cintia Carella; Sahoko Matsuoka; Tiphaine Bouriez Jones; Onima Chowdhury; Laura Stenson; Michael Lutteropp; Joanna C A Green; Raffaella Facchini; Hanane Boukarabila; Amit Grover; Adriana Gambardella; Supat Thongjuea; Joana Carrelha; Paul Tarrant; Deborah Atkinson; Sally-Ann Clark; Claus Nerlov; Sten Eirik W Jacobsen
Journal:  Nature       Date:  2013-08-11       Impact factor: 49.962

4.  IL-1β inhibits ILC3 while favoring NK-cell maturation of umbilical cord blood CD34(+) precursors.

Authors:  Paolo Ambrosini; Fabrizio Loiacono; Romana Conte; Lorenzo Moretta; Chiara Vitale; Maria Cristina Mingari
Journal:  Eur J Immunol       Date:  2015-05-12       Impact factor: 5.532

5.  Silencing of ASXL1 impairs the granulomonocytic lineage potential of human CD34⁺ progenitor cells.

Authors:  Carwyn Davies; Bon Ham Yip; Marta Fernandez-Mercado; Petter S Woll; Xabier Agirre; Felipe Prosper; Sten E Jacobsen; James S Wainscoat; Andrea Pellagatti; Jacqueline Boultwood
Journal:  Br J Haematol       Date:  2013-01-08       Impact factor: 6.998

6.  Myeloid differentiation of human cord blood CD34+ cells during ex vivo expansion using thrombopoietin, flt3-ligand and/or granulocyte-colony stimulating factor.

Authors:  E S Yoo; K H Ryu; H Y Park; C M Seong; W S Chung; S C Kim; Y M Choi; M J Hahn; S Y Woo; J Y Seoh
Journal:  Br J Haematol       Date:  1999-06       Impact factor: 6.998

7.  Ordering human CD34+CD10-CD19+ pre/pro-B-cell and CD19- common lymphoid progenitor stages in two pro-B-cell development pathways.

Authors:  Eva Sanz; Norman Muñoz-A; Jorge Monserrat; Ana Van-Den-Rym; Pedro Escoll; Ismael Ranz; Melchor Alvarez-Mon; Antonio de-la-Hera
Journal:  Proc Natl Acad Sci U S A       Date:  2010-03-15       Impact factor: 11.205

8.  Enforced expression of an Flt3 internal tandem duplication in human CD34+ cells confers properties of self-renewal and enhanced erythropoiesis.

Authors:  Ki Young Chung; Giovanni Morrone; Jan Jacob Schuringa; Bryan Wong; David C Dorn; Malcolm A S Moore
Journal:  Blood       Date:  2004-07-08       Impact factor: 22.113

9.  Characteristics of hematopoietic stem cells of umbilical cord blood.

Authors:  Anna Hordyjewska; Łukasz Popiołek; Anna Horecka
Journal:  Cytotechnology       Date:  2014-11-06       Impact factor: 2.058

10.  Identification of a potent small molecule capable of regulating polyploidization, megakaryocyte maturation, and platelet production.

Authors:  Nick Huang; Mabel Lou; Hua Liu; Cecilia Avila; Yupo Ma
Journal:  J Hematol Oncol       Date:  2016-12-08       Impact factor: 17.388
View more
  1 in total

1.  Single-cell RNA Seq reveals cellular landscape-specific characteristics and potential etiologies for adolescent idiopathic scoliosis.

Authors:  Yilin Yang; Mingyuan Yang; Dongliang Shi; Kai Chen; Jian Zhao; Shisheng He; Yushu Bai; Pinquan Shen; Haijian Ni
Journal:  JOR Spine       Date:  2021-12-08
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.