Aoxin Li1, Hanwen Wu2, Yuanjiao Feng3, Siyu Deng1, Aijiao Hou1, Fenfang Che1, Yuanyuan Liu1, Qianqian Geng4, Hanwen Ni4, Yun Wei1. 1. State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, China. 2. Graham Centre for Agricultural Innovation (NSW Department of Primary Industries and Charles Sturt University), Wagga Wagga Agricultural Institute, NSW Department of Primary Industries, Wagga Wagga, NSW, Australia. 3. College of Agriculture, South China Agricultural University, Guangzhou, China. 4. Plant Protection College, China Agricultural University, Beijing, China.
Abstract
BACKGROUND: To enhance discovering efficiency of new herbicidal compounds, a strategy for rapidly screening out strongly herbicidal chemical components from natural resources is necessary. RESULTS: Seventeen essential oils selected from 14 Eucalyptus species and hybrids were evaluated for their herbicidal activities on annual ryegrass. A feasible strategy was established for rapidly pinpointing the chemicals contributing to strong herbicidal activities without the process of isolation and purification of individual compounds. This strategy was based on gas chromatography-mass spectrometry (GC-MS) coupled with principal component analysis (PCA) and verified by the bioassay results using several individual compounds. Two strong herbicidal compounds trans-pinocarveol and α-terpineol were screened out using the strategy. The strong herbicidal activity of trans-pinocarveol was discovered for the first time. CONCLUSION: The established strategy of screening herbicidal compounds from natural resources is simple, feasible and reliable.
BACKGROUND: To enhance discovering efficiency of new herbicidal compounds, a strategy for rapidly screening out strongly herbicidal chemical components from natural resources is necessary. RESULTS: Seventeen essential oils selected from 14 Eucalyptus species and hybrids were evaluated for their herbicidal activities on annual ryegrass. A feasible strategy was established for rapidly pinpointing the chemicals contributing to strong herbicidal activities without the process of isolation and purification of individual compounds. This strategy was based on gas chromatography-mass spectrometry (GC-MS) coupled with principal component analysis (PCA) and verified by the bioassay results using several individual compounds. Two strong herbicidal compounds trans-pinocarveol and α-terpineol were screened out using the strategy. The strong herbicidal activity of trans-pinocarveol was discovered for the first time. CONCLUSION: The established strategy of screening herbicidal compounds from natural resources is simple, feasible and reliable.