| Literature DB >> 31444762 |
Nisreen Shumayrikh1, Dipankar Sen2,3.
Abstract
Catalytic DNAs (DNAzymes) with peroxidase-like activity have great potential in bioanalytical chemistry [1], owing to numerous advantages that DNA enzymes offer over conventional protein enzymes, including structural simplicity, low cost, thermal stability, and straightforward handling and preparation. Maximizing the efficiency of the peroxidase activity of such DNAzymes is a subject in need of review. In this chapter, we discuss the optimal experimental conditions for the peroxidase activity of these DNAzymes and describe general procedures for their utilization.Keywords: ABTS; Amplex Red; DNAzyme; Guanine quadruplex; HRP; Heme; Hemin; Peroxidase activity
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Year: 2019 PMID： 31444762 DOI： 10.1007/978-1-4939-9666-7_22
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745