| Literature DB >> 31444755 |
Shuntaro Takahashi1, Naoki Sugimoto2,3.
Abstract
The formation of guanine-quadruplexes (G4s) in genomic DNA and RNA inhibits replication, transcription, and translation. Although several regions of the human genome exhibit G4-forming potential, the stability and topology of the G4s formed vary depending on the molecular environment. Here, we describe a protocol to quantitatively analyze the inhibitory effects of G4s with different stabilities and topologies on replication in conditions of molecular crowding.Entities:
Keywords: Circular dichroism; Gel electrophoresis; Kinetic analysis; Molecular crowding; Replication; Thermodynamics
Mesh：
Year: 2019 PMID： 31444755 DOI： 10.1007/978-1-4939-9666-7_15
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745