Jianguo Xu1, Zilong Zhang1, Qing Chen2, Lin Yang3, Jiao Yin4. 1. Department of Oncology Surgery, People's Hospital of Qinghai Province, Xining City, 810007, Qinghai Province, China. 2. Department of Orthopedic, The 991 Hospital of PLA, Nanjing City, 441011, Jiangsu Province, China. 3. Department of Immunity, Medical College of Hubei University of Arts and Science, No. 296 Longzhong Road, Xiangyang City, 441053, Hubei Province, China. 4. Department of Immunity, Medical College of Hubei University of Arts and Science, No. 296 Longzhong Road, Xiangyang City, 441053, Hubei Province, China. zetmmagnvxn57@163.com.
Abstract
BACKGROUND/AIMS: The purpose of this study is to explore the inhibition or activation effects of microRNA-146 B on the expression of PTP1B in gastric cancer cells. METHODS: The expressions of PTP1B and miR-146b in gastric cancer were detected by RT-qPCR. The effects of miR-146b on cell apoptosis and proliferation of gastric cancer were detected. The methods used in the detection process included Annexin V/PI dying method, colony formation assay, and MTT assay. The downstream target gene miR-146b was predicted and screened by bioinformatics and luciferase reporter assay. The mRNA and protein expressions of the target gene PTP1B miR-146b were determined using RT-qPCR and western blot. The expression of miR-146 B in mice was detected by the cells transfected with microRNA-146 B in vivo. RESULTS: Compared with normal tissues, PTP1B was higher and miR-146b was lower in cancer cells. Over-expression of miR-146b can inhibit cell viability and increase the apoptosis rate. According to the luciferase reporter assay, PTP1B was the downstream target gene of miR-146b. The re-introduction of PTP1B reversed the growth inhibition and apoptosis of gastric cancer cells induced by miR-146b. From the mouse xenograft model, the over-expression of miR-146b inhibited the tumor growth and reduced the expression level of PTP1B. CONCLUSION: miR-146b directly inhibits the expression of PTP1B and suppressed the growth and development of gastric cancer.
BACKGROUND/AIMS: The purpose of this study is to explore the inhibition or activation effects of microRNA-146 B on the expression of PTP1B in gastric cancer cells. METHODS: The expressions of PTP1B and miR-146b in gastric cancer were detected by RT-qPCR. The effects of miR-146b on cell apoptosis and proliferation of gastric cancer were detected. The methods used in the detection process included Annexin V/PI dying method, colony formation assay, and MTT assay. The downstream target gene miR-146b was predicted and screened by bioinformatics and luciferase reporter assay. The mRNA and protein expressions of the target gene PTP1BmiR-146b were determined using RT-qPCR and western blot. The expression of miR-146 B in mice was detected by the cells transfected with microRNA-146 B in vivo. RESULTS: Compared with normal tissues, PTP1B was higher and miR-146b was lower in cancer cells. Over-expression of miR-146b can inhibit cell viability and increase the apoptosis rate. According to the luciferase reporter assay, PTP1B was the downstream target gene of miR-146b. The re-introduction of PTP1B reversed the growth inhibition and apoptosis of gastric cancer cells induced by miR-146b. From the mouse xenograft model, the over-expression of miR-146b inhibited the tumor growth and reduced the expression level of PTP1B. CONCLUSION:miR-146b directly inhibits the expression of PTP1B and suppressed the growth and development of gastric cancer.
Authors: J R Wiener; J A Hurteau; B J Kerns; R S Whitaker; M R Conaway; A Berchuck; R C Bast Journal: Am J Obstet Gynecol Date: 1994-04 Impact factor: 8.661