Rapid and simultaneous determination of three fluoroquinolones in animal-derived foods using excitation-emission matrix fluorescence coupled with second-order calibration method.

The matter of fluoroquinolone residues in various foods still arouses wide public concern nowadays. In the present work, the strategy of excitation-emission matrix (EEM) fluorescence data coupled with second-order calibration method based on alternating normalization-weighted error (ANWE) algorithm was used to determine ofloxacin, lomefloxacin and ciprofloxacin in milk powder, milk and beef. Owning the unique "second-order advantage", the ANWE-assisted analytical method was proved to successfully and eco-friendly resolve the overlapped fluorescence spectra of multi-component in complex food matrixes without tedious pretreatment steps and sophisticated high-cost instrumentations. The feasibility of the proposed method was validated by experiments. The average spiked recoveries of three fluoroquinolones range from 82.6% to 110.5% with relative standard deviations lower than 7.4%, and the limits of detection range from 0.18 and 2.41 ng mL-1. For further evaluation, analytical figures of merit such as sensitivity and selectivity, as well as the RSDs of intra-day (≦10.6%) and inter-day (≦9.4%) were calculated. The satisfactory analytical results demonstrated that the proposed strategy could be a competitive alternative for simple, rapid and simultaneous determination of multiple fluoroquinolones in animal-derived food samples.