Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Cloning, sequencing, and expression of cDNA for human beta-galactosidase.

Literature DB >> 3143362

Cloning, sequencing, and expression of cDNA for human beta-galactosidase.

A Oshima¹, A Tsuji, Y Nagao, H Sakuraba, Y Suzuki.

Abstract

We cloned and sequenced the full-length cDNA for human placental beta-galactosidase. The 2379-nucleotide sequence contains 2031 nucleotides which encode a protein of 677 amino acids. The amino acid sequence includes a putative signal sequence of 23 amino acids and 7 potential asparagine-linked glycosylation sites. The cDNA in the expression vector pSVL was used to transfect COS cells. Expression of the cDNA in transfected COS cells produced immunoprecipitable proteins and led to an increase in beta-galactosidase activity.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1988 PMID： 3143362 DOI： 10.1016/s0006-291x(88)80038-x

Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575

Keyword Cloud
Cited

30 in total

1. Characterization of beta-galactosidase mutations Asp332-->Asn and Arg148-->Ser, and a polymorphism, Ser532-->Gly, in a case of GM1 gangliosidosis.

Authors: S Zhang; R Bagshaw; W Hilson; Y Oho; A Hinek; J T Clarke; J W Callahan
Journal: Biochem J Date: 2000-06-15 Impact factor: 3.857

2. Characterization of an exo-beta-D-glucosaminidase involved in a novel chitinolytic pathway from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1.

Authors: Takeshi Tanaka; Toshiaki Fukui; Haruyuki Atomi; Tadayuki Imanaka
Journal: J Bacteriol Date: 2003-09 Impact factor: 3.490

10. Chaperone therapy for neuronopathic lysosomal diseases: competitive inhibitors as chemical chaperones for enhancement of mutant enzyme activities.

Authors: Yoshiyuki Suzuki; Seiichiro Ogawa; Yasubumi Sakakibara
Journal: Perspect Medicin Chem Date: 2009-05-26

Cloning, sequencing, and expression of cDNA for human beta-galactosidase.

1. Characterization of beta-galactosidase mutations Asp332-->Asn and Arg148-->Ser, and a polymorphism, Ser532-->Gly, in a case of GM1 gangliosidosis.

2. Characterization of an exo-beta-D-glucosaminidase involved in a novel chitinolytic pathway from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1.

3. Expression, purification, crystallization and preliminary X-ray crystallographic analysis of human β-galactosidase.

4. Modulating action of the new polymorphism L436F detected in the GLB1 gene of a type-II GM1 gangliosidosis patient.

5. Mutations in the lysosomal beta-galactosidase gene that cause the adult form of GM1 gangliosidosis.

6. Molecular consequences of the pathogenic mutation in feline GM1 gangliosidosis.

7. Mutations in acid beta-galactosidase cause GM1-gangliosidosis in American patients.

8. Assignment of human beta-galactosidase-A gene to 3p21.33 by fluorescence in situ hybridization.

9. Human beta-galactosidase gene mutations in GM1-gangliosidosis: a common mutation among Japanese adult/chronic cases.

10. Chaperone therapy for neuronopathic lysosomal diseases: competitive inhibitors as chemical chaperones for enhancement of mutant enzyme activities.