G Perrot1, C Colin-Pierre1, L Ramont2, I Proult3, C Garbar4, V Bardey5, C Jeanmaire5, S Mine5, L Danoux5, N Berthélémy5, F X Maquart3, Y Wegrowski3, S Brézillon6. 1. CNRS UMR 7369, Université de Reims Champagne-Ardenne, Faculté de Médecine, 51 rue Cognacq Jay, 51095 Reims, France; BASF Beauty Care Solutions France S.A.S, 3 rue de seichamps, 54425 Pulnoy, France. 2. CNRS UMR 7369, Université de Reims Champagne-Ardenne, Faculté de Médecine, 51 rue Cognacq Jay, 51095 Reims, France; CHU de Reims, Laboratoire Central de Biochimie, avenue du Général Koenig, 51092 Reims, France. 3. CNRS UMR 7369, Université de Reims Champagne-Ardenne, Faculté de Médecine, 51 rue Cognacq Jay, 51095 Reims, France. 4. Institut Jean Godinot, 1 rue du Général Koenig, 51100 Reims, France. 5. BASF Beauty Care Solutions France S.A.S, 3 rue de seichamps, 54425 Pulnoy, France. 6. CNRS UMR 7369, Université de Reims Champagne-Ardenne, Faculté de Médecine, 51 rue Cognacq Jay, 51095 Reims, France. Electronic address: stephane.brezillon@univ-reims.fr.
Abstract
BACKGROUND: Glypicans (GPCs) are heparan sulfate cell membrane proteoglycans containing glycosylphosphatidylinositol (GPI) anchor. They play important role in cell behavior by activating/presenting numerous growth factors and cytokines. OBJECTIVES: The expression of GPCs was investigated in primary culture of skin keratinocytes sampled from healthy donors of different age. MATERIALS AND METHODS: Primary keratinocytes from healthy female donors aged from 20 to 89 years old (n = 30) were either isolated from breast or abdominal skin samples (n = 27) or purchased (n = 3). GPCs expression was examined by qPCR, immunohistochemistry and western blot. Its role in proliferation induced by fibroblast growth factor 2 (FGF2) was also studied. RESULTS: Glypican 1 (GPC1) was the major expressed GPC in human keratinocytes. Its expression was up to two orders of magnitude higher than other GPCs and was significantly decreased with the age of the donors. It was localized at the cell surface and associated with intracellular granules. In skin sections, GPC1 was mainly localized in basal layer of epidermis. Shedding of GPCs decreased the proliferative effect of FGF2, confirming their role of modulator of growth factor effects on keratinocytes. These results established GPC1 as an important player in epidermis biology and skin ageing.
BACKGROUND: Glypicans (GPCs) are heparan sulfate cell membrane proteoglycans containing glycosylphosphatidylinositol (GPI) anchor. They play important role in cell behavior by activating/presenting numerous growth factors and cytokines. OBJECTIVES: The expression of GPCs was investigated in primary culture of skin keratinocytes sampled from healthy donors of different age. MATERIALS AND METHODS: Primary keratinocytes from healthy female donors aged from 20 to 89 years old (n = 30) were either isolated from breast or abdominal skin samples (n = 27) or purchased (n = 3). GPCs expression was examined by qPCR, immunohistochemistry and western blot. Its role in proliferation induced by fibroblast growth factor 2 (FGF2) was also studied. RESULTS:Glypican 1 (GPC1) was the major expressed GPC in human keratinocytes. Its expression was up to two orders of magnitude higher than other GPCs and was significantly decreased with the age of the donors. It was localized at the cell surface and associated with intracellular granules. In skin sections, GPC1 was mainly localized in basal layer of epidermis. Shedding of GPCs decreased the proliferative effect of FGF2, confirming their role of modulator of growth factor effects on keratinocytes. These results established GPC1 as an important player in epidermis biology and skin ageing.
Authors: Charlie Colin-Pierre; Nicolas Berthélémy; Nicolas Belloy; Louis Danoux; Vincent Bardey; Romain Rivet; Solène Mine; Christine Jeanmaire; François-Xavier Maquart; Laurent Ramont; Stéphane Brézillon Journal: Front Cell Dev Biol Date: 2021-12-08