Haihan Jiao1, Cecilia Naranjo Golborne2, Samantha J Dando3,4, Paul G McMenamin4, Laura E Downie1, Holly R Chinnery1. 1. Department of Optometry and Vision Sciences, The University of Melbourne , Melbourne, Australia. 2. Monash Genome Modification Platform, Faculty of Medicine, Nursing and Health Sciences, Monash University , Melbourne, Australia. 3. School of Biomedical Sciences and Institute of Health and Biomedical Innovation, Queensland University of Technology , Brisbane, Australia. 4. Monash Biomedicine Discovery Institute and Department of Anatomy & Developmental Biology, Faculty of Medicine, Nursing and Health Sciences, Monash University , Melbourne, Australia.
Abstract
PURPOSE: We report novel differences in mouse corneal DC morphology and density during local and systemic inflammation. METHODS: Local inflammation was induced by topical application of saline or TLR9 agonist CpG-ODN on abraded C57BL6J mouse corneas. Systemic inflammation was induced by intraperitoneal injection of lipopolysaccharide (LPS) in CD11c-YFP mice. Corneal epithelial DCs from uninjured, injured and contralateral eyes were analysed by confocal microscopy. RESULTS: Following local CpG delivery on the injured cornea, the DC density and size increased in both central and peripheral regions. Contralateral uninjured eyes displayed enlarged DC morphology in the central cornea compared to naïve cohorts. After systemic LPS, the size of DCs in the central cornea was lower at 2 hours, returning to baseline after 24 hours. CONCLUSIONS: Corneal DCs respond differently in terms of shape and distribution during local and systemic inflammation. These features can serve as in vivo indicators in ocular and systemic diseases.
PURPOSE: We report novel differences in mouse corneal DC morphology and density during local and systemic inflammation. METHODS: Local inflammation was induced by topical application of saline or TLR9 agonist CpG-ODN on abraded C57BL6J mouse corneas. Systemic inflammation was induced by intraperitoneal injection of lipopolysaccharide (LPS) in CD11c-YFP mice. Corneal epithelial DCs from uninjured, injured and contralateral eyes were analysed by confocal microscopy. RESULTS: Following local CpG delivery on the injured cornea, the DC density and size increased in both central and peripheral regions. Contralateral uninjured eyes displayed enlarged DC morphology in the central cornea compared to naïve cohorts. After systemic LPS, the size of DCs in the central cornea was lower at 2 hours, returning to baseline after 24 hours. CONCLUSIONS: Corneal DCs respond differently in terms of shape and distribution during local and systemic inflammation. These features can serve as in vivo indicators in ocular and systemic diseases.
Entities:
Keywords:
Cornea; dendritic cells; local inflammation; systemic inflammation
Authors: Mengliang Wu; Laura E Downie; Liam M Grover; Richard J A Moakes; Saaeha Rauz; Ann Logan; Haihan Jiao; Lisa J Hill; Holly R Chinnery Journal: J Neuroinflammation Date: 2020-05-04 Impact factor: 8.322
Authors: Haihan Jiao; Laura E Downie; Xin Huang; Mengliang Wu; Sara Oberrauch; Ryan J Keenan; Laura H Jacobson; Holly R Chinnery Journal: J Neuroinflammation Date: 2020-04-28 Impact factor: 8.322
Authors: Cirous Dehghani; Shaun Frost; Rajiv Jayasena; Christopher Fowler; Colin L Masters; Yogesan Kanagasingam; Haihan Jiao; Jeremiah K H Lim; Holly R Chinnery; Laura E Downie Journal: Front Neurosci Date: 2020-12-09 Impact factor: 4.677