Literature DB >> 3142934

An ELISA to detect antipseudomonal IgA antibodies in sera of patients with cystic fibrosis.

M M Brett1, A T Ghoneim, J M Littlewood.   

Abstract

An enzyme linked immunosorbent assay (ELISA) to measure free serum IgA antibodies to Pseudomonas aeruginosa in patients with cystic fibrosis is described. Results were reproducible and there was no interference from crossreacting antibodies directed against other Gram negative bacteria. Titres were high in patients with Pseudomonas aeruginosa infection and differed according to the stage of the infection. These preliminary results suggest that this assay may be of value in assessing the state of Pseudomonas aeruginosa infection in patients with cystic fibrosis.

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Year:  1988        PMID： 3142934      PMCID： PMC1141703          DOI： 10.1136/jcp.41.10.1130

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  11 in total

1.  A test for concentration of electrolytes in sweat in cystic fibrosis of the pancreas utilizing pilocarpine by iontophoresis.

Authors:  L E GIBSON; R E COOKE
Journal:  Pediatrics       Date:  1959-03       Impact factor: 7.124

2.  The enzyme-linked immunosorbent assay (ELISA): a measure of antibody concentration or affinity.

Authors:  J E Butler; T L Feldbush; P L McGivern; N Stewart
Journal:  Immunochemistry       Date:  1978-02

Review 3.  Expression of immune mechanisms in the lung.

Authors:  H B Kaltreider
Journal:  Am Rev Respir Dis       Date:  1976-03

Review 4.  Pulmonary disease associated with Pseudomonas aeruginosa in cystic fibrosis: current status of the host-bacterium interaction.

Authors:  G B Pier
Journal:  J Infect Dis       Date:  1985-04       Impact factor: 5.226

5.  Rapid diagnosis of Pseudomonas aeruginosa urinary tract infections by radioimmunoassay.

Authors:  R B Kohler; L J Wheat; A White
Journal:  J Clin Microbiol       Date:  1979-02       Impact factor: 5.948

6.  Serum IgG antibodies in patients with cystic fibrosis with early Pseudomonas aeruginosa infection.

Authors:  M M Brett; A T Ghoneim; J M Littlewood
Journal:  Arch Dis Child       Date:  1987-04       Impact factor: 3.791

7.  Eosinophilic infiltrates of the gastrointestinal tract.

Authors:  A J Blackshaw; D A Levison
Journal:  J Clin Pathol       Date:  1986-01       Impact factor: 3.411

8.  Measurement of human antibody activity against Escherichia coli and Pseudomonas aeruginosa using formalin treated whole organisms in an ELISA technique.

Authors:  K M Cost; C S West; D Brinson; H C Polk
Journal:  J Immunoassay       Date:  1985

9.  Lung defense against infection.

Authors:  P G Quie
Journal:  J Pediatr       Date:  1986-05       Impact factor: 4.406

10.  Prospective study of serum antibodies to Pseudomonas aeruginosa exoproteins in cystic fibrosis.

Authors:  A E Hollsing; M Granström; M L Vasil; B Wretlind; B Strandvik
Journal:  J Clin Microbiol       Date:  1987-10       Impact factor: 11.677
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  3 in total

1.  Serum IgA antibodies against Pseudomonas aeruginosa in cystic fibrosis.

Authors:  M M Brett; A T Ghoneim; J M Littlewood
Journal:  Arch Dis Child       Date:  1990-03       Impact factor: 3.791

2.  Immunoglobulin A and immunoglobulin G antibody responses to alginates from Pseudomonas aeruginosa in patients with cystic fibrosis.

Authors:  S S Pedersen; F Espersen; N Høiby; T Jensen
Journal:  J Clin Microbiol       Date:  1990-04       Impact factor: 5.948

3.  IgG antibodies in early Pseudomonas aeruginosa infection in cystic fibrosis.

Authors:  S M Cordon; J S Elborn; R J Rayner; E J Hiller; D J Shale
Journal:  Arch Dis Child       Date:  1992-06       Impact factor: 3.791
  3 in total

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