| Literature DB >> 31427757 |
Jeongmin Kim1,2, Michal Wojcik3, Yuan Wang1,2, Seonah Moon3, Emilia A Zin4, Nadia Marnani5, Zachary L Newman5, John G Flannery4,5, Ke Xu6,7,8, Xiang Zhang9,10.
Abstract
Single-molecule localization microscopy (SMLM), while well established for cultured cells, is not yet fully compatible with tissue-scale samples. We introduce single-molecule oblique-plane microscopy (obSTORM), which by directly imaging oblique sections of samples with oblique light-sheet illumination offers a deep and volumetric SMLM platform that is convenient for standard tissue samples and small intact animals. We demonstrate super-resolution imaging at depths of up to 66 µm for cells, Caenorhabditis elegans gonads, Drosophila melanogaster larval brain, mouse retina and brain sections, and whole stickleback fish.Entities:
Mesh：
Year: 2019 PMID： 31427757 DOI： 10.1038/s41592-019-0510-z
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547