Literature DB >> 31424632

OMIP-061: 20-Color Flow Cytometry Panel for High-Dimensional Characterization of Murine Antigen-Presenting Cells.

Anthony T DiPiazza1, Juliane P Hill2, Barney S Graham1, Tracy J Ruckwardt1.   

Abstract

This 20-color flow cytometry panel was designed to resolve the cellular heterogeneity of antigen-presenting cells and was optimized for lymph node tissue. Reagents were carefully selected and optimized for identification of B cells (B220), neutrophils (Ly6G), monocytes and macrophages (Ly6C, CD169, F4/80), and dendritic cells (XCR1, CD172a, CD11c, I-A/I-E, CD24, CD64, pDCA-1, CD103, CD11b). Inclusion of additional functional markers involved in cell migration (CCR7), co-stimulation (CD80), and adhesion (ICAM-1) enabled further phenotypic characterization. Finally, this panel has been tested and is compatible with fluorescently labeled antigens such as Alexa Fluor 488 (Ax488) for the study of antigen-bearing cells in vivo. Published 2019. This article is a U.S. Government work and is in the public domain in the USA. Published 2019. This article is a U.S. Government work and is in the public domain in the USA.

Entities:  

Keywords:  antigen-presenting cells; dendritic cells; immunophenotyping; lymph node; macrophages; mouse; myeloid cells

Mesh：

Substances：

Year:  2019        PMID： 31424632     DOI： 10.1002/cyto.a.23880

Source DB:  PubMed          Journal:  Cytometry A        ISSN： 1552-4922            Impact factor:   4.355


  8 in total

1.  Broad immunophenotyping of the murine brain tumor microenvironment.

Authors:  W H Tomaszewski; J Waibl-Polania; A M Miggelbrink; M A Chakraborty; P E Fecci; J H Sampson; M D Gunn
Journal:  J Immunol Methods       Date:  2021-09-29       Impact factor: 2.303

2.  Mouse splenocyte enrichment strategies via negative selection for broadened single-cell transcriptomics.

Authors:  Thomas T Schulze; Andrew J Neville; Ryan C Chapman; Paul H Davis
Journal:  STAR Protoc       Date:  2022-05-17

3.  The cytosolic tryparedoxin peroxidase from Trypanosoma cruzi induces a pro-inflammatory Th1 immune response in a peroxidatic cysteine-dependent manner.

Authors:  Lucía López; María Laura Chiribao; Magalí C Girard; Karina A Gómez; Paula Carasi; Marisa Fernandez; Yolanda Hernandez; Carlos Robello; Teresa Freire; María Dolores Piñeyro
Journal:  Immunology       Date:  2021-01-25       Impact factor: 7.397

4.  SARS-CoV-2 ferritin nanoparticle vaccine induces robust innate immune activity driving polyfunctional spike-specific T cell responses.

Authors:  Joshua M Carmen; Shikha Shrivastava; Zhongyan Lu; Alexander Anderson; Elaine B Morrison; Rajeshwer S Sankhala; Wei-Hung Chen; William C Chang; Jessica S Bolton; Gary R Matyas; Nelson L Michael; M Gordon Joyce; Kayvon Modjarrad; Jeffrey R Currier; Elke Bergmann-Leitner; Allison M W Malloy; Mangala Rao
Journal:  NPJ Vaccines       Date:  2021-12-13       Impact factor: 7.344

5.  Single-cell transcriptome analysis reveals the immune heterogeneity and the repopulation of microglia by Hif1α in mice after spinal cord injury.

Authors:  Jingyu Wang; Lintao Xu; Weiwei Lin; Yin Yao; Heyangzi Li; Gerong Shen; Xi Cao; Ning He; Jun Chen; Jue Hu; Mingzhi Zheng; Xinghui Song; Yuemin Ding; Yueliang Shen; Jinjie Zhong; Lin-Lin Wang; Ying-Ying Chen; Yongjian Zhu
Journal:  Cell Death Dis       Date:  2022-05-03       Impact factor: 9.685

6.  Assay for ADAMTS-13 Activity with Flow Cytometric Readout.

Authors:  Jens Müller; Nasim Shahidi Hamedani; Hannah L McRae; Heiko Rühl; Johannes Oldenburg; Bernd Pötzsch
Journal:  ACS Omega       Date:  2022-08-24

7.  Acquisition of murine splenic myeloid cells for protein and gene expression profiling by advanced flow cytometry and CITE-seq.

Authors:  Inga Rødahl; James Gotley; Stacey B Andersen; Meihua Yu; Ahmed M Mehdi; Angelika N Christ; Emma E Hamilton-Williams; Ian H Frazer; Samuel W Lukowski; Janin Chandra
Journal:  STAR Protoc       Date:  2021-09-17

8.  OMIP 076: High-dimensional immunophenotyping of murine T-cell, B-cell, and antibody secreting cell subsets.

Authors:  Kyle T Mincham; Jacob D Young; Deborah H Strickland
Journal:  Cytometry A       Date:  2021-06-23       Impact factor: 4.714
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.