Differential pathlength factor in continuous wave functional near-infrared spectroscopy: reducing hemoglobin's cross talk in high-density recordings.

Functional near-infrared spectroscopy (fNIRS) estimates the functional oscillations of oxyhemoglobin and deoxyhemoglobin in the cortex through scalp-located multiwavelength recordings. Hemoglobin oscillations are inferred through temporal changes in continuous-wave (CW) light attenuation. However, because of the diffusive multilayered head tissue structures, the photon path is longer than the source-detector separation, complicating hemoglobin evaluation. This aspect is incorporated in the modified Beer-Lambert law where the source-detector distance is multiplied by the differential pathlength factor (DPF). Since DPF estimation requires photons' time-of-flight information, DPF is assumed a priori in CW-fNIRS. Importantly, errors in the DPF spectrum induce hemoglobin cross talk, which is detrimental for fNIRS. We propose to estimate subject-specific DPF spectral dependence relying on multidistance high-density measurements. The procedure estimates the effective attenuation coefficient (EAC), which is proportional to the geometric mean of absorption and reduced scattering. Since DPF depends on the scattering-to-absorption ratio, EAC limits the spectral dependence assumption to scattering. This approach was compared to a standard frequency-domain multidistance procedure. A good association between the two methods ( r 2 = 0.69 ) was obtained. This approach could estimate low-resolution maps of the DPF spectral dependence through large field of view, high-density systems, reducing hemoglobin cross talk, and increasing fNIRS sensitivity and specificity to brain activity without instrumentation modification.