Literature DB >> 3142303

Precipitation and recovery of proteins from culture supernatants using zinc.

P G Zaworski1, G S Gill.   

Abstract

A convenient method for the concentration of proteins from culture supernatants is described. Heterologous gene products expressed and secreted into the culture medium by Saccharomyces cerevisiae, Chinese hamster ovary cells (CHO), and Bacillus subtilis were precipitated out of solution by the addition of millimolar concentrations of zinc chloride. Porcine urokinase secreted by S. cerevisiae, the tissue-type plasminogen activator analog FK2P secreted by CHO cells, and human interleukin-1 beta secreted by B. subtilis were all precipitated by zinc ions. Both urokinase and FK2P were precipitated with as low as 2 mM zinc, with recovery of activity in the precipitate approaching 100%.

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Year:  1988        PMID： 3142303     DOI： 10.1016/0003-2697(88)90211-4

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  7 in total

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Authors:  Slawomir A Dziedzic; Allan B Caplan
Journal:  Autophagy       Date:  2011-05-01       Impact factor: 16.016

2.  Effect of recombinant human lecithin cholesterol acyltransferase infusion on lipoprotein metabolism in mice.

Authors:  Xavier Rousset; Boris Vaisman; Bruce Auerbach; Brian R Krause; Reyn Homan; John Stonik; Gyorgy Csako; Robert Shamburek; Alan T Remaley
Journal:  J Pharmacol Exp Ther       Date:  2010-07-06       Impact factor: 4.030

3.  Enhanced protein export in Saccharomyces cerevisiae nud1 mutants is an active process.

Authors:  M G Pesheva; M K Koprinarova; P Venkov
Journal:  Curr Microbiol       Date:  2006-10-26       Impact factor: 2.188

4.  A 17-kDa Nicotiana tabacum cell-wall peptide acts as an in-vitro inhibitor of the cell-wall isoform of acid invertase.

Authors:  M Weil; S Krausgrill; A Schuster; T Rausch
Journal:  Planta       Date:  1994       Impact factor: 4.116

5.  Expression of high levels of human tissue plasminogen activator in yeast under the control of an inducible GAL promoter.

Authors:  E Martegani; N Forlani; I Mauri; D Porro; W D Schleuning; L Alberghina
Journal:  Appl Microbiol Biotechnol       Date:  1992-08       Impact factor: 4.813

6.  Continuous capture of recombinant antibodies by ZnCl 2 precipitation without polyethylene glycol.

Authors:  Gregory Dutra; Daniel Komuczki; Alois Jungbauer; Peter Satzer
Journal:  Eng Life Sci       Date:  2020-03-24       Impact factor: 2.678

7.  Metal Ion Promiscuity and Structure of 2,3-Dihydroxybenzoic Acid Decarboxylase of Aspergillus oryzae.

Authors:  Gerhard Hofer; Xiang Sheng; Simone Braeuer; Stefan E Payer; Katharina Plasch; Walter Goessler; Kurt Faber; Walter Keller; Fahmi Himo; Silvia M Glueck
Journal:  Chembiochem       Date:  2020-11-23       Impact factor: 3.164
  7 in total

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