Gang Wang1, Diana N Ionescu1, Cheng-Han Lee1, Tadaaki Hiruki1, Renelle Myers2, Tawimas Shaipanich2, Stephen Lam2, Barbara Melosky3, Chen Zhou4. 1. Department of Pathology, BC Cancer Vancouver Centre, University of British Columbia, Vancouver, BC, Canada. 2. Department of Integrative Oncology, BC Cancer Research Center, University of British Columbia, Vancouver, BC, Canada. 3. Department of Medical Oncology, BC Cancer Vancouver Centre, University of British Columbia, Vancouver, BC, Canada. 4. Department of Pathology, BC Cancer Vancouver Centre, University of British Columbia, Vancouver, BC, Canada. Electronic address: czhou@bccancer.bc.c.
Abstract
OBJECTIVES: The FDA approved PD-L1 tests for anti-PD-L1 immunotherapy are for surgical or histology specimens. It is not clear if cytology specimens could be used for PD-L1 testing to guide immunotherapy. In this study, we assess the suitability of EBUS-FNA cytology specimens for the testing of PD-L1. MATERIALS AND METHODS: Consecutive patients with Non-small cell lung cancer (NSCLC) underwent EBUS procedure between January 1, 2017 and March 31, 2018 for PD-L1 testing were included. The cell blocks of EBUS-FNA cytology specimens were used for PD-L1 testing using Dako 22C3 phamDx antibody according to the Dako protocol. PD-L1 protein expression in tumor cells is determined by using Tumor Proportion Score (TPS). RESULTS AND CONCLUSION: Of the 265 EBUS-FNA specimens from 262 patients sent for testing, 230 (86.8%) were adequate for PD-L1 testing. Of the 34 NSCLC patients with both histology and EBUS-FNA cytology specimens tested for PD-L1, the results from different specimen types had a concordance of 91.3%. The PD-L1 results from 16 paired specimens from the same anatomic site had 100% agreement. The rates of PD-L1 TPS ≥ 50% were significantly higher in the metastatic tumors in the lymph nodes than in the lung primary lesions. Therefore, EBUS-FNA cytology specimen is suitable for PD-L1 testing in patients with advanced NSCLC. The metastatic tumors in mediastinal lymph nodes appear to have higher PD-L1 expression than primary lesions.
OBJECTIVES: The FDA approved PD-L1 tests for anti-PD-L1 immunotherapy are for surgical or histology specimens. It is not clear if cytology specimens could be used for PD-L1 testing to guide immunotherapy. In this study, we assess the suitability of EBUS-FNA cytology specimens for the testing of PD-L1. MATERIALS AND METHODS: Consecutive patients with Non-small cell lung cancer (NSCLC) underwent EBUS procedure between January 1, 2017 and March 31, 2018 for PD-L1 testing were included. The cell blocks of EBUS-FNA cytology specimens were used for PD-L1 testing using Dako 22C3 phamDx antibody according to the Dako protocol. PD-L1 protein expression in tumor cells is determined by using Tumor Proportion Score (TPS). RESULTS AND CONCLUSION: Of the 265 EBUS-FNA specimens from 262 patients sent for testing, 230 (86.8%) were adequate for PD-L1 testing. Of the 34 NSCLCpatients with both histology and EBUS-FNA cytology specimens tested for PD-L1, the results from different specimen types had a concordance of 91.3%. The PD-L1 results from 16 paired specimens from the same anatomic site had 100% agreement. The rates of PD-L1 TPS ≥ 50% were significantly higher in the metastatic tumors in the lymph nodes than in the lung primary lesions. Therefore, EBUS-FNA cytology specimen is suitable for PD-L1 testing in patients with advanced NSCLC. The metastatic tumors in mediastinal lymph nodes appear to have higher PD-L1 expression than primary lesions.
Authors: Mari Mino-Kenudson; Nolwenn Le Stang; Jillian B Daigneault; Andrew G Nicholson; Wendy A Cooper; Anja C Roden; Andre L Moreira; Erik Thunnissen; Mauro Papotti; Giuseppe Pelosi; Noriko Motoi; Claudia Poleri; Elisabeth Brambilla; Mary Redman; Deepali Jain; Sanja Dacic; Yasushi Yatabe; Ming Sound Tsao; Fernando Lopez-Rios; Johan Botling; Gang Chen; Teh-Ying Chou; Fred R Hirsch; Mary Beth Beasley; Alain Borczuk; Lukas Bubendorf; Jin-Haeng Chung; David Hwang; Dongmei Lin; John Longshore; Masayuki Noguchi; Natasha Rekhtman; Lynette Sholl; William Travis; Akihiko Yoshida; Murry W Wynes; Ignacio I Wistuba; Keith M Kerr; Sylvie Lantuejoul Journal: J Thorac Oncol Date: 2021-03-02 Impact factor: 20.121
Authors: Mohammed S I Mansour; Kajsa Ericson Lindquist; Tomas Seidal; Ulrich Mager; Rikard Mohlin; Lena Tran; Kim Hejny; Benjamin Holmgren; Despoina Violidaki; Katalin Dobra; Annika Dejmek; Maria Planck; Hans Brunnström Journal: Acta Cytol Date: 2021-07-07 Impact factor: 2.319