Young-Seok Lee1, Dae-Chul Cho2, Chi Heon Kim3, Inbo Han4, Eun Young Gil2, Kyoung-Tae Kim5. 1. Department of Neurosurgery, Gyeongsang National University, Gyeongsang National University Hospital, Jinju, Republic of Korea. 2. Department of Neurosurgery, School of Medicine, Kyungpook National University, Kyungpook National University Hospital, Daegu, Republic of Korea. 3. Department of Neurosurgery, Seoul National University Hospital, Seoul, Republic of Korea; Department of Neurosurgery, Seoul National University College of Medicine, 103 Daehak-ro, Jongno-gu, 03080 Seoul, South Korea. 4. Department of Neurosurgery, CHA University, CHA Bundang Medical Center, Seongnam-si, Gyeonggido, Republic of Korea. 5. Department of Neurosurgery, School of Medicine, Kyungpook National University, Kyungpook National University Hospital, Daegu, Republic of Korea. Electronic address: nskimkt7@gmail.com.
Abstract
BACKGROUND CONTEXT: Curcumin has anti-inflammatory and antioxidant activities. OBJECTIVE: This study aimed to investigate the effects of curcumin on the histological changes and functional recovery following spinal cord injury (SCI). STUDY DESIGN: One hundred twenty-eight Sprague-Dawley rats were distributed into a sham, SCI only, SCI-hyperglycemia, and SCI-hyperglycemia-curcumin (200 mg/kg/day, i.p.) groups. METHODS: SCI was induced using a clip at T9-10 and hyperglycemia was induced by streptozotocin (60-70 mg/kg, i.v.). Plasma malondialdehyde levels and superoxide dismutase activity was measured to determine oxidative stress. The activity of macrophages in the spinal cord after SCI was stained by the anti-CD68 antibody (ED-1). The tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-8 levels were measured by enzyme-linked immunosorbent assay and Western blot was used to verify the levels of mitogen-activated protein kinases and STAT3. The glial fibrillary acidic protein expression was evaluated by immunofluorescence analysis. Functional recovery was assessed according to the Basso, Beattie, and Bresnahan scale and histologic outcome was evaluated by the lesion volume and spared tissue area. RESULTS: Superoxide dismutase activity increased, the malondialdehyde level decreased, and ED-1 macrophage marker level decreased in the SCI-hyperglycemia-curcumin group than in the SCI-hyperglycemia group at 2 weeks after SCI (p<.01). The SCI-hyperglycemia-curcumin group showed a statistically significant reduction in IL-6, IL-8, and TNF-α levels compared with the SCI-hyperglycemia group after SCI. The phosphorylated-extracellular signal-regulated kinase, phosphorylated-JNK, and phospho-p38 levels were significantly lower in the SCI-hypoglycemia-curcumin group than in the SCI-hypoglycemia group. The SCI-hyperglycemia-curcumin group showed a decrease in glial fibrillary acidic protein expression after SCI compared with the SCI-hyperglycemia group. The SCI-hyperglycemia-curcumin group showed a lower lesion volume, higher spared tissue, and better functional recovery than the SCI-hyperglycemia group. CONCLUSIONS: Curcumin may have a potential neuroprotective effect in SCI with hyperglycemia. CLINICAL SIGNIFICANCE: Curcumin decreased the inflammatory response and decreased astrogliosis and improved the functional recovery and histologic outcomes in SCI with hyperglycemia.
BACKGROUND CONTEXT: Curcumin has anti-inflammatory and antioxidant activities. OBJECTIVE: This study aimed to investigate the effects of curcumin on the histological changes and functional recovery following spinal cord injury (SCI). STUDY DESIGN: One hundred twenty-eight Sprague-Dawley rats were distributed into a sham, SCI only, SCI-hyperglycemia, and SCI-hyperglycemia-curcumin (200 mg/kg/day, i.p.) groups. METHODS: SCI was induced using a clip at T9-10 and hyperglycemia was induced by streptozotocin (60-70 mg/kg, i.v.). Plasma malondialdehyde levels and superoxide dismutase activity was measured to determine oxidative stress. The activity of macrophages in the spinal cord after SCI was stained by the anti-CD68 antibody (ED-1). The tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-8 levels were measured by enzyme-linked immunosorbent assay and Western blot was used to verify the levels of mitogen-activated protein kinases and STAT3. The glial fibrillary acidic protein expression was evaluated by immunofluorescence analysis. Functional recovery was assessed according to the Basso, Beattie, and Bresnahan scale and histologic outcome was evaluated by the lesion volume and spared tissue area. RESULTS:Superoxide dismutase activity increased, the malondialdehyde level decreased, and ED-1 macrophage marker level decreased in the SCI-hyperglycemia-curcumin group than in the SCI-hyperglycemia group at 2 weeks after SCI (p<.01). The SCI-hyperglycemia-curcumin group showed a statistically significant reduction in IL-6, IL-8, and TNF-α levels compared with the SCI-hyperglycemia group after SCI. The phosphorylated-extracellular signal-regulated kinase, phosphorylated-JNK, and phospho-p38 levels were significantly lower in the SCI-hypoglycemia-curcumin group than in the SCI-hypoglycemia group. The SCI-hyperglycemia-curcumin group showed a decrease in glial fibrillary acidic protein expression after SCI compared with the SCI-hyperglycemia group. The SCI-hyperglycemia-curcumin group showed a lower lesion volume, higher spared tissue, and better functional recovery than the SCI-hyperglycemia group. CONCLUSIONS:Curcumin may have a potential neuroprotective effect in SCI with hyperglycemia. CLINICAL SIGNIFICANCE: Curcumin decreased the inflammatory response and decreased astrogliosis and improved the functional recovery and histologic outcomes in SCI with hyperglycemia.
Authors: Anna Lucia Fedullo; Mario Ciccotti; Paolo Giannotta; Federica Alviti; Marco Bernardi; Anna Raguzzini; Elisabetta Toti; Tommaso Sciarra; Ilaria Peluso Journal: Oxid Med Cell Longev Date: 2021-02-27 Impact factor: 6.543
Authors: Ahmet Yardım; Fatih Mehmet Kandemir; Selim Çomaklı; Selçuk Özdemir; Cuneyt Caglayan; Sefa Kucukler; Hamit Çelik Journal: Neurochem Res Date: 2020-11-17 Impact factor: 3.996