Leucaena leucocephala (Lam.) galactomannan nanoparticles: Optimization and characterization for ocular delivery in glaucoma treatment.

In the present study, bioadhesive polymeric nanoparticles for ocular delivery of dorzolamide hydrochloride (DRZ) were formulated using derivatized Leucaena leucocephala galactomannan (LLG). The carboxymethyl (CMLLG) and amine derivative (AMLLG) of LLG were synthesized and used for the preparation of nanoparticles. The preparation method was optimized using 2-factor, 3-level central composite experimental design. The optimized nanoparticles were characterized by FTIR, DSC, SEM, TEM and XRD studies. The results of in vitro release studies and ex vivo transcorneal permeation studies revealed sustained release and higher corneal permeation as compared to conventional formulation of DRZ. The optimized nanoparticles were found to reduce the intra ocular pressure (IOP) for prolonged period of time when compared to conventional eye drops. Overall, the present study suggests a promising role of bioadhesive polymeric nanoparticles for ocular drug delivery in glaucoma treatment.