Correction: Perfluorooctane Sulfonate Disturbs Nanog Expression through miR-490-3p in Mouse Embryonic Stem Cells.

[This corrects the article DOI: 10.1371/journal.pone.0074968.].

In Fig 2b, the image appearing in the GADPH row is incorrect. Please see the complete, correct Fig 2 here.

Fig 2

Effects of PFOS on pluripotency and expressions of miR-145, miR-490-3p in mESCs.

Cells were cultured with various concentrations of PFOS (0.2 μM, 2 μM, 20 μM, and 200 μM) or DMSO as control for 24 h. (A) Oct-4/Sox-2/Nanog mRNA levels were determined by quantitative real-time PCR using a housekeeping gene GAPDH as an internal control. (B) The protein levels of Oct-4/Sox-2/Nanog were determined by Western blot analysis using GAPDH as an internal control. (C) miRNA levels (miR-145, miR-490-3p) were determined by quantitative real-time PCR and were normalized to U6 as an internal control. Each data point was normalized to the control (DMSO) and represented the means ± S.E. from three independent experiments. (D) Relative protein levels of Oct4, Sox2 and Nanog. *indicates significant difference when the values were compared to that of the control (p<0.05).