The influence of endodontic treatment on blood pressure reduction in patients with vital irreversible pulpitis.

BACKGROUND: During endodontic treatment, endodontists must be aware of the various factors that may decrease or increase blood pressure. This study aimed to assess the mean percentages of systolic, diastolic, and arterial blood pressure (MSBP, MDAP, MABP) reduction in patients with vital irreversible pulpitis in teeth and who were treated at three visits to endodontists in three age groups (20-34 years, 35-50 years, 51-65 years).
MATERIALS AND METHODS: A total of 100 teeth with vital irreversible pulpitis from 100 patients were included. All patients underwent 3 visits for endodontic treatment. The 1st visit included removal of vital pulp tissue and a determination of working length, the 2nd visit included canal preparation and widening, and at the 3rd visit the canal was obturated and sealed by gutta percha and sealer. Blood pressure for all patients was checked and documented once before starting treatment and three times during treatment at different intervals during all visits.
RESULTS: There were significantly higher percentage reductions in MSBP, MDBP, and MABP at the 1st visit for endodontic treatment in comparison to other visits (2nd and 3rd) for all patients. Additionally, there were significantly higher percentage reductions in MSBP, MDBP, and MABP at the 1st visit for endodontic treatment in comparison to other visits in males and females, for all age groups, both anesthesia injection-type groups (infiltration and block), and all treated tooth types except mandibular anterior teeth, and there were nonsignificant differences among groups. However, there were nonsignificant differences in MSBP, MDBP, and MABP between males and females, between infiltration and block injection groups and in relation to teeth types at all visits.
CONCLUSIONS: The reduction of blood pressure in patients undergoing endodontic treatment of vital teeth with irreversible pulpits is common, especially at the 1st visit for pulp extirpation.

Introduction

An increase in blood pressure (BP) is considered an important risk factor that may lead to death in patients around the world.1–3 A change in BP (increase or decrease) is related directly to the sensation of pain or anxiety in patients. This pain can serve as an alarm to the body to prevent damage to tissues, and the affected area can send signals to activate the sympathetic nervous system (SNS) through neurophysiological linkages. This can lead to increased heart rate and adrenal gland stimulation, which can result in an increase in BP. However, if the pain disappears, the same area sends another signal to activate the parasympathetic nervous system (PNS), which leads to decreased heart rate and hypotension.4–6

During dental treatment, especially endodontic treatment, the endodontist must know how to deal with healthy and compromised patients and how to complete root canal procedures successfully and without complications.3

Throughout the access-opening appointment, pain or anxiety may cause in activation of the SNS, leading to changes in heart rate and BP. This can be due to the release of endogenous catecholamines (adrenaline and noradrenaline) and not due to the effect of exogenous catecholamines found in local anesthetics.7,8 However, other authors have suggested that this change in BP or heart rate may be due to adrenaline present in local anesthetic solutions, which plays a role in prolonging the time that local anesthesia is effective and may cause unwanted side effects, such as increasing BP and heart rate as the vasoconstricting effect of adrenaline leads to increased contraction of blood vessels and increased blood flow.9,10 Pereira, in 2013,11 discussed the effect of using local anesthesia with different concentrations of adrenaline at single-visit endodontic treatments of molars with pulpitis and found that adrenaline did not lead to significant alterations in heart rate or systolic and diastolic BP under clinical conditions. In 2008, Liau et al.12 explained the effect of both tooth extraction and local anesthesia (in different age groups) on the activation of internal catecholamines and changes in their concentrations in the body during extraction. Additionally, other studies have investigated the effect of performing restorations with and without local anesthesia.6

In this study, we recorded and analyzed BP measurements before and during root canal treatment at different time periods in the teeth of Iraqi patients with vital irreversible pulpitis treated over three visits. We assessed the mean percentage reductions of systolic blood pressure (SBP), diastolic blood pressure (DBP), and arterial blood pressure (ABP).

Materials and methods

In this study, we recruited 100 Iraqi patients (50 males, 50 females) from Baghdad with irreversible pulpitis in three age groups (group 1: 20–34 years, 31 patients; group 2: 35–50 years, 22 patients; group 3: 51–65 year, 47 patients). A written consent form was obtained from all patients for blood pressure measurements, root canal treatment agreement and publishing data. This study was approved by the Ethics Committee of Al-Rafidain University-College. All patients underwent complete root canal treatment (3 visits) under local anesthesia with 2% lidocaine with 1:100,000 epinephrine (1.7 ml). The injection technique was either infiltration or inferior dental blocking according to the treated tooth.

The vitality of pulp was checked before giving anesthesia using a pulp tester and ethyl chloride spray; only vital teeth were selected for this study. Patients with systemic diseases, pregnancy, or mental retardation, those who where heavy smokers (more than 10 cig/day), those who taking any medication for pain, and those with their 3rd molar teeth were excluded from the study. Anesthesia was given at each visit. All root canal treatments during each visit were performed with rubber dam isolation and by same endodontist. In the access-opening visit (1st visit), the vital pulp tissue was removed using a barbed broach, and the working length was determined by hand file (initial size) with the aid of an Apex locator (iPex II-NSK, Japan) and digital X-ray sensor (RVG 6100 Carestream-Kodak, USA). Then, irrigation was performed with 2.5% sodium hypochlorite (NaOCl) and finally with normal saline, after which the canals were dried with paper points. Finally, teeth were medicated with calcium hydroxide paste (Metapex-META BIOMED) applied in the root canals, and teeth were sealed with a temporary filling.

In the instrumentation or preparation visit (2nd visit), complete preparation of the root canal system was performed using Gold ProTaper rotary files from Dentsply with a Proglider. Repeated 2.5% NaOCl irrigation was performed throughout the sequence of filing, and the final irrigation was with normal saline. Then, the canal was dried with a paper point and sealed with sterile cotton and a temporary filling.

In the obturation visit (3rd visit), the length of the canal was checked again using a digital X-ray sensor and irrigated with 2.5% NaOCl and then with normal saline. The canal was then dried with a paper point, and the canals were obturated by gutta-percha cones (Dentsply) with a sealer (Apexit Plus-Ivoclar Vivadent) using a cold lateral condensation technique.

The technique used for accurately determining BP included seating the patient in a chair for approximately 5 mins before measurement, with the patient’s feet on the floor in front of the chair and their hands on the chair arms. An upper-arm BP monitor (Beurer BM 58, Germany) was used in this study, and the cuff holder encircled at least 80% of the patient’s upper arm.1,13,14

During each visit, both SBP and DBP were measured four times for each patient. The initial measurement (1st measurement) was taken before starting the treatment (before giving anesthesia). The minimum measurement (measured during treatment) represents the lowest value from three measurements taken during the treatment as follows: 1st measurement: measured 10 mins after starting treatment.

Second measurement: measured 20 mins after starting treatment.

Third measurement: measured 30 mins after starting treatment.

The mean arterial pressure was calculated using the following equation:

Mean SBP (MSBP), mean DBP (MDBP), and mean ABP (MABP) were calculated from the following equations:

Patients with SBP higher than 140 mmHg or DBP lower than 90 mmHg (at rest) were excluded from the study.

Data were analyzed using SPSS software, and descriptive statistics, analysis of variance (ANOVA), least difference analysis (LSD) and Independent t-tests were used in our study. The results with P-values less than 0.01 are highly significant (HS), and the results with P-values between 0.01 and 0.04 are significant (S), while P-values equal to or greater than 0.05 represent nonsignificant (NS) results.

Results

This study included 100 patients, each with a tooth with irreversible pulpitis. Fifty teeth were selected from males, and fifty teeth were selected from females. The patients’ ages ranged from 20 to 65 year: group 1 (20–34 years) included 31% of the patients, group 2 (35–50 years) included 22%, and group 3 (51–65 years) included 47%.

Maxillary and mandibular anterior teeth represented 6% of all the teeth. Maxillary premolars represented 19% of the teeth, mandibular premolars 14%, maxillary molars 28%, and mandibular molars 27%. Fifty-two percent of teeth with pulpitis were treated under local infiltration anesthesia, while 48% were treated under inferior dental block anesthesia.

The MSBP percentage reductions were 8.45, 5.38, and 3.11 at the 1st, 2nd, and 3rd visits, respectively, the MDBP percentage reductions were 11.75, 6.45, and 3.27 at the 1st, 2nd, and 3rd visits, respectively, and the MABP percentage reductions were 10.21, 6.10, and 3.39 at the 1st, 2nd, and 3rd visits, respectively. The percentage reductions in MSBP, MDBP, and MABP were significantly higher at the 1st visit for root canal treatment than at both the 2nd and 3rd visits for all treated patients. Additionally, there were highly significant differences in MSBP, MDBP, and MABP between the 1st and 2nd visits, 1st and 3rd visits, and 2nd and 3rd visits. Table 1

Table 1

Comparison of MSBP, MDBP, and MABP reduction percentages among patients receiving three endodontic treatment visits

Descriptive					ANOVA test		LSD test
MBP	Visits	Teeth No.	Mean	SD	F-test	P-value	Visits	P-value
MSBP	First visit	100	8.45	5.40	47.61	0.000/HS	First visit X second visit	0.000/HS
	Second visit	100	5.38	3.22			First visit X third visit	0.000/HS
Third visit	100	3.11	2.36	Second visit X third visit	0.000/HS
MDBP	First visit	100	11.75	8.75	56.75	0.000/HS	First visit X second visit	0.000/HS
	Second visit	100	6.45	3.95			First visit X third visit	0.000/HS
Third visit	100	3.27	2.17	Second visit X third visit	0.000/HS
MABP	First visit	100	10.21	6.52	66.16	0.000/HS	First visit X second visit	0.000/HS
	Second visit	100	6.10	2.83			First visit X third visit	0.000/HS
Third visit	100	3.39	1.66	Second visit X third visit	0.000/HS

The participants were separated into 2 or more groups according to different clinical variables or factors (gender, age, tooth type, anesthesia type). We found that the percentage reductions in MSBP, MSBP, and MABP at the 1st visit were significantly higher than those at the 2nd and 3rd visits in males and females from all three age groups (group 1: 20–34, group 2: 35–50, group 3: 51–65), regardless of the anesthesia (infiltration or block) or tooth type except for mandibular anterior teeth, in which case mean reduction at the 1st visit was higher than that at other visits but the difference was not significant. Tables 2–5

Table 2

Comparison and independent t-test of MSBP, MDBP, and MABP reduction percentages among patients receiving three endodontic treatment visits according to patients’ gender

Descriptive						ANOVA test		LSD test
Gender	MBP	Visits	Teeth No.	Mean	SD	F-test	P-value	Visits	P-value
Males	MSBP	First visit	50	8.95	6.12	21.98	0.000/HS	First visit X second visit	0.000/HS
		Second visit	50	5.37	2.72			First visit X third visit	0.000/HS
	Third visit	50	3.45	2.84	Second visit X third visit	0.024/S
	MDBP	First visit	50	12.17	9.35	25.87	0.000/HS	First visit X second visit	0.000/HS
		Second visit	50	7.15	4.02			First visit X third visit	0.000/HS
	Third visit	50	3.50	2.49	Second visit X third visit	0.003/HS
	MABP	First visit	50	10.71	7.30	28.47	0.000/HS	First visit X second visit	0.000/HS
		Second visit	50	6.50	2.95			First visit X third visit	0.000/HS
Third visit	50	3.69	1.91	Second visit X third visit	0.003/HS
Females	MSBP	First visit	50	7.9	4.57	26.82	0.000/HS	First visit X second visit	0.000/HS
		Second visit	50	5.40	3.68			First visit X third visit	0.000/HS
	Third visit	50	2.77	1.73	Second visit X third visit	0.000/HS
	MDBP	First visit	50	11.34	8.18	31.62	0.000/HS	First visit X second visit	0.000/HS
		Second visit	50	5.76	3.80			First visit X third visit	0.000/HS
	Third visit	50	3.04	1.80	Second visit X third visit	0.012/S
	MABP	First visit	50	9.71	5.66	40.47	0.000/HS	First visit X second visit	0.000/HS
		Second visit	50	5.69	2.68			First visit X third visit	0.000/HS
Third visit	50	3.09	1.33	Second visit X third visit	0.003/HS
Independent t-test test for MBP reduction percentages between males and females.
Visits			MBP						Sig.
First, second, third			MSBP, MDBP, MABP						NS

Table 5

Comparison and independent t-test of MSBP, MDBP, and MABP reduction percentages among patients receiving three endodontic treatment visits according to type of anaesthesia injection

Descriptive						ANOVA test		LSD test
Anaesthesia	MBP	Visits	Teeth No.	Mean	SD	F-test	P-value	Visits	P-value
Infiltration	MSBP	First visit	52	8.97	6.23	21.73	0.000/HS	First visit X second visit	0.000/HS
		Second visit	52	5.44	3.43			First visit X third visit	0.000/HS
Third visit	52	3.32	2.77	Second visit X third visit	0.015/S
	MDBP	First visit	52	12.40	9.42	30.41	0.000/HS	First visit X second visit	0.000/HS
		Second visit	52	6.78	3.97			First visit X third visit	0.000/HS
Third visit	52	3.27	2.07	Second visit X third visit	0.003/HS
	MABP	First visit	52	10.78	7.28	33.81	0.000/HS	First visit X second visit	0.000/HS
		Second visit	52	6.19	2.74			First visit X third visit	0.000/HS
Third visit	52	3.43	1.73	Second visit X third visit	0.003/HS
Block	MSBP	First visit	48	7.89	4.31	28.97	0.000/HS	First visit X second visit	0.000/HS
		Second visit	48	5.32	3.01			First visit X third visit	0.000/HS
	Third visit	48	2.89	1.83	Second visit X third visit	0.000/HS
	MDBP	First visit	48	11.05	8.01	26.22	0.000/HS	First visit X second visit	0.000/HS
		Second visit	48	6.10	3.94			First visit X third visit	0.000/HS
	Third visit	48	3.28	2.30	Second visit X third visit	0.010/S
	MABP	First visit	48	9.59	5.59	33.05	0.000/HS	First visit X second visit	0.000/HS
		Second visit	48	5.99	2.96			First visit X third visit	0.000/HS
Third visit	48	3.35	1.60	Second visit X third visit	0.001/HS
Independent t-test test for MBP reduction percentages between infiltration and block.
Visits				MBP					Sig.
First, second, third				MSBP, MDBP, MABP					NS

Moreover, the percentage reductions in MSBP, MDBP, and MABP were higher in males than in females at all visits but were not significantly different. Additionally, there were highly significant differences in MSBP, MDBP, and MABP between the 1st and 2nd visits, 1st and 3rd visits, and 2nd and 3rd visits, and there was a significant difference in MSBP between the 2nd and 3rd visits in males and in MDBP between the 2nd and 3rd visits in females. Table 2

Statistically, there were nonsignificant differences in the percentage reductions in MSBP, MDBP, and MABP among the three age groups at all visits except for MDBP at the 1st visit, where there was a significant difference. Additionally, there were highly significant differences in MSBP, MDBP, and MABP between the 1st and 2nd visits and the 1st and 3rd visits for group 1. In group 2, there were highly significant differences in MDBP and MABP between the 1st and 2nd visits; however, for MSBP, there was a nonsignificant difference. There were significant differences in MDBP and MABP between the 2nd and 3rd visits, but for MSBP, the differences were highly significant. In group 3, there was a highly significant difference in MSBP between the 1st and 2nd visits, 1st and 3rd visits, and 2nd and 3rd visits, but not between the 2nd and 3rd visits. Table 3

Table 3

Comparison and ANOVA test of MSBP, MDBP, and MABP reduction percentages among visits in aged groups

Descriptive						ANOVA test		LSD test
Gender	MBP	Visits	Teeth No.	Mean	SD	F-test	P-value	Visits	P-value
G1 (20–34)	MSBP	First visit	31	9.71	7.01	19.18	0.000/HS	First visit X second visit	0.000/HS
		Second visit	31	4.61	2.83			First visit X third visit	0.000/HS
	Third visit	31	2.92	1.87	Second visit X third visit	0.142/NS
	MDBP	First visit	31	13.50	9.87	22.19	0.000/HS	First visit X second visit	0.000/HS
		Second visit	31	5.71	4.18			First visit X third visit	0.000/HS
	Third visit	31	5.44	3.03	Second visit X third visit	0.136/NS
	MABP	First visit	31	11.69	8.18	22.89	0.000/HS	First visit X second visit	0.000/HS
		Second visit	31	3.30	2.02			First visit X third visit	0.000/HS
Third visit	31	3.21	1.53	Second visit X third visit	0.090/NS
G2(35–50)	MSBP	First visit	22	7.69	4.78	10.63	0.000/HS	First visit X second visit	0.884/NS
		Second visit	22	7.51	4.40			First visit X third visit	0.000/HS
	Third visit	22	2.79	2.37	Second visit X third visit	0.000/HS
	MDBP	First visit	22	14.21	9.44	17.59	0.000/HS	First visit X second visit	0.000/HS
		Second visit	22	7.12	4.42			First visit X third visit	0.000/HS
	Third visit	22	3.36	2.28	Second visit X third visit	0.047/S
	MABP	First visit	22	11.44	7.12	18.10	0.000/HS	First visit X second visit	0.001/HS
		Second visit	22	6.51	2.53			First visit X third visit	0.000/HS
Third visit	22	3.36	1.86	Second visit X third visit	0.023/S
G3 (51–65)	MSBP	First visit	47	7.98	4.32	24.57	0.000/HS	First visit X second visit	0.000/HS
		Second visit	47	4.90	2.34			First visit X third visit	0.000/HS
	Third visit	47	3.40	2.65	Second visit X third visit	0.025/S
	MDBP	First visit	47	9.45	7.08	20.18	0.000/HS	First visit X second visit	0.005/HS
		Second visit	47	6.63	3.56			First visit X third visit	0.000/HS
	Third visit	47	3.22	2.26	Second visit X third visit	0.001/HS
	MABP	First visit	47	8.65	4.48	30.01	0.000/HS	First visit X second visit	0.001/HS
		Second visit	47	6.34	2.82			First visit X third visit	0.000/HS
Third visit	47	3.53	1.68	Second visit X third visit	0.000/HS
ANOVA test for MBP reduction percentages among aged groups.
Visits				MBP					Sig.
First				MSBP, MABP					NS
MDBP					S
Second and third				MSBP, MDBP, MABP					NS

There were nonsignificant differences in the percentage reductions in MSBP, MDBP, and MABP among the 6 teeth-type groups at all visits. Additionally, in the maxillary anterior teeth group, there were highly significant differences in MSBP, MDBP, and MABP between the 1st and 2nd visits and in MSBP and MABP between the 1st and 3rd visits in group 1; MDBP was not significantly different between the 1st and 3rd visits. Additionally, there were nonsignificant differences in MSBP and MDBP between the 2nd and 3rd visits, whereas the difference in MABP was significant. In the group of mandibular anterior teeth, there were nonsignificant differences in MSBP, MDBP, MABP between the 1st and 2nd and 2nd and 3rd visits, but there were significant differences between MDBP and MABP at the 1st and 3rd visits. In the maxillary premolar group, there were highly significant differences between MDBP and MABP at the 1st and 2nd and the 1st and 3rd visits; for MSBP, the difference was highly significant between the 1st and 3rd visits and significant between the 1st and 2nd visits. In addition, there were nonsignificant differences in MDBP and MABP and a significant difference in MSBP between the 2nd and 3rd visits. In mandibular premolars, there were highly significant differences in MSBP, MDBP, and MABP between the 1st and 2nd, 1st and 3rd, and 2nd and 3rd visits except for the differences in MDBP and MABP between the 1st and 2nd visits, which were not significant. There were also nonsignificant differences in MSBP between the 2nd and 3rd visits. In the maxillary molar group, there were significant differences in MSBP and MDBP between the 1st and 2nd visits, but there was a highly significant difference in MABP. There was also a highly significant difference in MSBP, MDBP, and MABP between the 1st and 3rd visits. In contrast, there was a nonsignificant difference in MSBP between the 2nd and 3rd visits but a highly significant difference in MDBP and a significant difference in MABP. In the mandibular molar group, there were highly significant differences in MSBP and MDBP between the 1st and 2nd, 1st and 3rd, and 2nd and 3rd visits, whereas for MABP, the difference was highly significant between the 1st and 2nd and the 1st and 3rd visits but not the 2nd and 3rd visits. Table 4

Table 4

Comparison and ANOVA test of MSBP, MDBP, and MABP reduction percentages among visits in each teeth type

Descriptive						ANOVA test		LSD test
Teeth types	MBP	Visits	Teeth No.	Mean	SD	F-test	P-value	Visits	P-value
Max. anterior	MSBP	First visit	6	11.53	6.78	9.72	0.002/HS	First visit X second visit	0.007/HS
		Second visit	6	4.28	0.62			First visit X third visit	0.001/HS
	Third visit	6	1.66	1.40	Second visit X third visit	0.277/NS
	MDBP	First visit	6	12.20	5.06	6.04	0.012/HS	First visit X second visit	0.170/NS
		Second visit	6	8.65	5.14			First visit X third visit	0.003/HS
	Third visit	6	3.68	1.58	Second visit X third visit	0.062/NS
	MABP	First visit	6	11.84	3.50	16.27	0.000/HS	First visit X second visit	0.006/HS
		Second visit	6	6.97	2.67			First visit X third visit	0.000/HS
Third visit	6	3.29	0.95	Second visit X third visit	0.027/S
Mand. anterior	MSBP	First visit	6	4.45	1.71	2.11	0.156/NS	First visit X second visit	0.465/NS
		Second visit	6	5.61	2.82			First visit X third visit	0.219/NS
	Third visit	6	2.45	3.29	Second visit X third visit	0.060/NS
	MDBP	First visit	6	6.07	2.72	2.72	0.098/NS	First visit X second visit	0.118/NS
		Second visit	6	4.04	1.55			First visit X third visit	0.040/S
	Third visit	6	3.31	1.91	Second visit X third visit	0.561/NS
	MABP	First visit	6	5.36	1.48	3.18	0.070/NS	First visit X second visit	0.076/NS
		Second visit	6	3.97	1.03			First visit X third visit	0.031/S
Third visit	6	3.55	1.88	Second visit X third visit	0.637/NS
Max. premolar	MSBP	First visit	19	9.33	6.12	9.61	0.000/HS	First visit X second visit	0.037/S
		Second visit	19	6.28	3.98			First visit X third visit	0.000/HS
	Third visit	19	3.08	2.08	Second visit X third visit	0.029/S
	MDBP	First visit	19	15.15	11.3	15.00	0.000/HS	First visit X second visit	0.000/HS
		Second visit	19	5.73	3.29			First visit X third visit	0.000/HS
	Third visit	19	3.60	1.87	Second visit X third visit	0.347/NS
	MABP	First visit	19	12.17	8.83	13.56	0.000/HS	First visit X second visit	0.000/HS
		Second visit	19	5.78	1.82			First visit X third visit	0.000/HS
Third visit	19	3.61	1.36	Second visit X third visit	0.209/NS
Mand. premolar	MSBP	First visit	14	8.20	4.05	13.17	0.000/HS	First visit X second visit	0.002/HS
		Second visit	14	4.79	2.15			First visit X third visit	0.000/HS
	Third visit	14	2.96	1.22	Second visit X third visit	0.085/NS
	MDBP	First visit	14	9.19	3.38	15.81	0.000/HS	First visit X second visit	0.038/S
		Second visit	14	7.04	2.04			First visit X third visit	0.000/HS
	Third visit	14	3.63	2.29	Second visit X third visit	0.001/HS
	MABP	First visit	14	8.80	2.94	20.81	0.000/HS	First visit X second visit	0.012/S
		Second visit	14	6.58	2.18			First visit X third visit	0.000/HS
Third visit	14	3.36	1.30	Second visit X third visit	0.001/HS
Max. molar	MSBP	First visit	28	8.11	6.14	6.94	0.002/HS	First visit X second visit	0.012/S
		Second visit	28	5.08	3.28			First visit X third visit	0.001/HS
	Third visit	28	3.81	3.22	Second visit X third visit	0.288/NS
	MDBP	First visit	28	10.73	8.31	14.15	0.000/HS	First visit X second visit	0.012/S
		Second visit	28	6.94	4.09			First visit X third visit	0.000/HS
	Third visit	28	2.89	2.28	Second visit X third visit	0.007/HS
	MABP	First visit	28	9.69	6.58	14.71	0.000/HS	First visit X second visit	0.004/HS
		Second visit	28	6.20	3.27			First visit X third visit	0.000/HS
Third visit	28	3.30	2.06	Second visit X third visit	0.016/S
Mand. molar	MSBP	First visit	27	8.52	4.64	16.67	0.000/HS	First visit X second visit	0.003/HS
		Second visit	27	5.63	3.46			First visit X third visit	0.000/HS
	Third visit	27	3.03	1.74	Second visit X third visit	0.008/HS
	MDBP	First visit	27	12.92	9.82	16.04	0.000/HS	First visit X second visit	0.000/HS
		Second visit	27	6.25	4.82			First visit X third visit	0.000/HS
	Third visit	27	3.16	2.44	Second visit X third visit	0.083/NS
	MABP	First visit	27	10.76	6.78	18.35	0.000/HS	First visit X second visit	0.000/HS
		Second visit	27	6.19	3.47			First visit X third visit	0.000/HS
Third visit	27	3.39	1.71	Second visit X third visit	0.025/S
ANOVA test for MBP reduction percentages among teeth in each visit.
Visits				MBP					Sig.
First, second, third				MSBP, MDBP, MABP					NS

Finally, the percentage reductions in MSBP, MDBP, and MABP were higher in the infiltration group than in the blocking group at all visits, but this difference was not significant. Table 5

Discussion

We chose patients whose ages ranged from 20–65 years because these ages represent the ages at which most patients undergo root canal treatment for maintaining their teeth. The 51–65 age group constituted 47% of the total sample, and this may be related to the fact that younger patients may have less time to go to endodontists and maintain their teeth; additionally, financial factors may form a barrier that prevents them from making endodontic visits, or it may be related to dental or endodontic anxiety.15–18

This study showed a decrease in BP during treatment (minimum measured BP) at all visits. This is due to the fact that after anesthetizing the tooth with pulpitis, the pain decreases and disappears gradually, and the brain sends signals to activate the PNS, which leads to a decrease in BP in patients.5,6

According to our study, the percentage reductions at the 1st visit were significantly higher than at the 2nd and 3rd visits for all groups of clinical variables (gender, age, tooth type, anesthesia type), except in the mandibular anterior group, where there were nonsignificant differences. This may be related to the fact that in access opening (1st visit), the vital pulp tissue and nerve were extirpated and removed, while at the 2nd visit, there was minimal remaining pulp tissue, so intervention with the dental nerve branches was reduced or nonexistent; at the 3rd visit, there was no remaining pulp tissue. In the lower anterior dentition, which are enervated by incisive nerves, there are few terminal divisions of the inferior alveolar nerves.19 The dental nerves of the lower anterior dentition are a long way from the trigeminal ganglion and brainstem and may contain the least sensory fibers when compared with the dental nerves of the other tooth types.20 Along these lines, with complete removal of vital pulp tissue in these teeth, the reduced degree of trigeminal induction may prompt a small PNS impact on lowering BP. In total, these results agreed with Huang et al in 2017,6 except in specific age groups where Huang et al showed nonsignificant results only for patients below 40 years of age. This may be related to differences in age group selection as Huang et al divided their sample into 6 groups (20–29, 30–39, 40–49, 50–59, and 60–69 years), while our study divided the sample into only 3 groups (20–34, 35–50, and 51–65 years).

The percentage reductions in BP were higher in males than in females at all visits but are not statistically significant, and this result agrees with Huang et al in 2017.6 This may be linked to the fact that females had greater average levels of anxiety than males.17,18 Therefore, the decreasing anxiety in males leads to activation of the PNS, which leads to a decrease in BP in males that is higher than that in females.

At the 1st visit, the percentage reductions in BP were higher in the 20–34 age group than in the other groups, while the 35–50 age group showed a higher reduction at the 2nd visit. At the 3rd visit, the 51–65 age group showed a higher reduction than the other groups. These findings might be credited to the way that younger adults are most likely to be more restless or stressed while getting endodontic treatment than are older patients, and consequently, a significant SNS reaction is evoked that lessens or masks the PNS impact on BP in patients undergoing root canal treatment. Additionally, there was a nonsignificant difference in the percentage reductions in MSBP, MDBP, and MABP among the three age groups at all visits except for MDBP at the 1st visit where there was a significant difference, which disagrees with Huang et al in 20176 which showed nonsignificant results at all visits. This may be related to differences in age group selection.

Moreover, at the 1st visit, the percentage reductions in BP were higher in the maxillary anterior and premolar groups than in the other groups, while the least reduction was is the mandibular anterior group. This might be connected to the fact that the anterior and middle superior alveolar nerves supply the maxillary anterior and premolar teeth sequentially and represent the main branches of the infraorbital nerve, whereas the lower anterior teeth are provided by the incisive nerves, which are separate nerves and are the small terminal divisions of the inferior alveolar nerves.19 Additionally, there were nonsignificant differences in the percentage reductions in MSBP, MDBP, and MABP among the six teeth-type groups at all visits, and this agrees with Huang et al in 2017.6

Finally, the percentage reductions in BP were higher in the infiltration group than in the blocking group at all visits, but the differences were not significant, and this result disagrees with Huang et al in 2017,6 which showed a higher percentage reduction in the block anesthesia group than in the infiltration group, with nonsignificant differences. Rogers et al in 201421 and Shapiro et al in 201822 were found that the success rate of anesthesia was increased with the using of both inferior dental blocking and supplemental buccal infiltration than inferior dental blocking only to anesthetized mandibular molars teeth with irreversible pulpitis during access opening visit. This may be related to the nature of the bone of the maxilla, which is cancellous and trabecular or spongy, while the mandibular bone is compact,23 so infiltration anesthesia is faster and more effective than blocking, and patients given blocking anesthesia are probably more anxious and fearful when undergoing root canal treatment than are patients receiving infiltration anesthesia, which positively influenced BP reduction in the infiltration group.24

Conclusion

The reduction of BP in patients undergoing endodontic treatment of vital teeth with irreversible pulpits is a generally basic event, especially at the access opening visit. Moreover, the reduction of BP in younger patients are higher than older patients in access opening visit. Also, the reduction of BP in males was higher than females and in the infiltration group was higher than block group.