Anne M Karvonen1, Pirkka V Kirjavainen2, Martin Täubel3, Balamuralikrishna Jayaprakash3, Rachel I Adams4, Joanne E Sordillo5, Diane R Gold6, Anne Hyvärinen3, Sami Remes7, Erika von Mutius8, Juha Pekkanen9. 1. Department of Health Security, Finnish Institute for Health and Welfare, Kuopio, Finland; Channing Division of Network Medicine, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Mass. Electronic address: anne.karvonen@thl.fi. 2. Department of Health Security, Finnish Institute for Health and Welfare, Kuopio, Finland; Institute of Public Health and Clinical Nutrition, University of Eastern Finland, Kuopio, Finland. 3. Department of Health Security, Finnish Institute for Health and Welfare, Kuopio, Finland. 4. Plant & Microbial Biology, University of California, Berkeley, Calif; California Department of Public Health, Environmental Health Laboratory Branch, Richmond, Calif. 5. Channing Division of Network Medicine, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Mass; Department of Population Medicine, Harvard Medical School and Harvard Pilgrim Health Care, Boston, Mass. 6. Channing Division of Network Medicine, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Mass. 7. Department of Pediatrics, Kuopio University Hospital, Kuopio, Finland. 8. Dr. von Hauner Children's Hospital, Ludwig-Maximilians-Universität, Munich, Germany; Member of the German Center for Lung Research, Giessen, Germany; Institute for Asthma and Allergy Prevention (IAP), Helmholtz Zentrum München, Munich, Germany. 9. Department of Health Security, Finnish Institute for Health and Welfare, Kuopio, Finland; Department of Public Health, University of Helsinki, Helsinki, Finland.
Abstract
BACKGROUND: Early-life indoor bacterial exposure is associated with the risk of asthma, but the roles of specific bacterial genera are poorly understood. OBJECTIVE: We sought to determine whether individual bacterial genera in indoor microbiota predict the development of asthma. METHODS: Dust samples from living rooms were collected at 2 months of age. The dust microbiota was characterized by using Illumina MiSeq sequencing amplicons of the bacterial 16S ribosomal RNA gene. Children (n = 373) were followed up for ever asthma until the age of 10.5 years. RESULTS: Richness was inversely associated with asthma after adjustments (P = .03). The phylogenetic microbiota composition in asthmatics patients' homes was characteristically different from that in nonasthmatic subjects' homes (P = .02, weighted UniFrac, adjusted association, permutational multivariate analysis of variance, PERMANOVA-S). The first 2 axis scores of principal coordinate analysis of the weighted UniFrac distance matrix were inversely associated with asthma. Of 658 genera detected in the dust samples, the relative abundances of 41 genera correlated (r > |0.4|) with one of these axes. Lactococcus genus was a risk factor for asthma (adjusted odds ratio, 1.36 [95% CI, 1.13-1.63] per interquartile range change). The abundance of 12 bacterial genera (mostly from the Actinomycetales order) was associated with lower asthma risk (P < .10), although not independently of each other. The sum relative abundance of these 12 intercorrelated genera was significantly protective and explained the majority of the association of richness with less asthma. CONCLUSION: Our data confirm that phylogenetic differences in the microbiota of infants' homes are associated with subsequent asthma risk and suggest that communities of selected bacteria are more strongly linked to asthma protection than individual bacterial taxa or mere richness.
BACKGROUND: Early-life indoor bacterial exposure is associated with the risk of asthma, but the roles of specific bacterial genera are poorly understood. OBJECTIVE: We sought to determine whether individual bacterial genera in indoor microbiota predict the development of asthma. METHODS: Dust samples from living rooms were collected at 2 months of age. The dust microbiota was characterized by using Illumina MiSeq sequencing amplicons of the bacterial 16S ribosomal RNA gene. Children (n = 373) were followed up for ever asthma until the age of 10.5 years. RESULTS: Richness was inversely associated with asthma after adjustments (P = .03). The phylogenetic microbiota composition in asthmatics patients' homes was characteristically different from that in nonasthmatic subjects' homes (P = .02, weighted UniFrac, adjusted association, permutational multivariate analysis of variance, PERMANOVA-S). The first 2 axis scores of principal coordinate analysis of the weighted UniFrac distance matrix were inversely associated with asthma. Of 658 genera detected in the dust samples, the relative abundances of 41 genera correlated (r > |0.4|) with one of these axes. Lactococcus genus was a risk factor for asthma (adjusted odds ratio, 1.36 [95% CI, 1.13-1.63] per interquartile range change). The abundance of 12 bacterial genera (mostly from the Actinomycetales order) was associated with lower asthma risk (P < .10), although not independently of each other. The sum relative abundance of these 12 intercorrelated genera was significantly protective and explained the majority of the association of richness with less asthma. CONCLUSION: Our data confirm that phylogenetic differences in the microbiota of infants' homes are associated with subsequent asthma risk and suggest that communities of selected bacteria are more strongly linked to asthma protection than individual bacterial taxa or mere richness.
Authors: Le Duc Huy Ta; Carina Jing Xuan Tay; Christophe Lay; Paola Florez de Sessions; Cheryl Pei Ting Tan; Michelle Jia Yu Tay; Hui Xing Lau; Atiqa Binte Zulkifli; Gaik Chin Yap; Elizabeth Huiwen Tham; Eliza Xin Pei Ho; Anne Eng Neo Goh; Keith M Godfrey; Johan G Eriksson; Jan Knol; Peter D Gluckman; Yap Seng Chong; Jerry Kok Yen Chan; Kok Hian Tan; Kok Wee Chong; Si Hui Goh; Zai Ru Cheng; Bee Wah Lee; Lynette Pei-Chi Shek; Evelyn Xiu Ling Loo Journal: Environ Microbiol Date: 2021-08-12 Impact factor: 5.476
Authors: Jenni M Mäki; Pirkka V Kirjavainen; Martin Täubel; Eija Piippo-Savolainen; Katri Backman; Anne Hyvärinen; Pauli Tuoresmäki; Balamuralikrishna Jayaprakash; Joachim Heinrich; Gunda Herberth; Marie Standl; Juha Pekkanen; Anne M Karvonen Journal: Sci Rep Date: 2021-03-05 Impact factor: 4.379
Authors: Matthias V Kopp; Cathleen Muche-Borowski; Michael Abou-Dakn; Birgit Ahrens; Kirsten Beyer; Katharina Blümchen; Petra Bubel; Adam Chaker; Monika Cremer; Regina Ensenauer; Michael Gerstlauer; Uwe Gieler; Inga-Marie Hübner; Fritz Horak; Ludger Klimek; Berthold V Koletzko; Sybille Koletzko; Susanne Lau; Thomas Lob-Corzilius; Katja Nemat; Eva M J Peters; Antonio Pizzulli; Imke Reese; Claudia Rolinck-Werninghaus; Elien Rouw; Bianca Schaub; Sebastian Schmidt; Jens-Oliver Steiß; Anne Kathrin Striegel; Zsolt Szépfalusi; Dietmar Schlembach; Thomas Spindler; Christian Taube; Valérie Trendelenburg; Regina Treudler; Ulrich Umpfenbach; Christian Vogelberg; Martin Wagenmann; Anke Weißenborn; Thomas Werfel; Margitta Worm; Helmut Sitter; Eckard Hamelmann Journal: Allergol Select Date: 2022-03-04