New selective medium for Pseudomonas aeruginosa with phenanthroline and 9-chloro-9-[4-(diethylamino)phenyl]-9,10-dihydro-10- phenylacridine hydrochloride (C-390).

A new selective medium (PC agar) for the isolation of Pseudomonas aeruginosa was developed, consisting of 30 micrograms of 9-chloro-9-[4-(diethylamino)phenyl]-9,10-dihydro-10-phenylacridine hydrochloride (C-390) per ml and 30 micrograms of phenanthroline per ml in Columbia agar. PC agar was superior to phenanthroline, C-390, acetamide, and cetrimide agars for the selective growth of P. aeruginosa.