Brunno Rf Verçoza1,2,3, Robson R Bernardo1,4, Arbélio Pentón-Madrigal5, João P Sinnecker6, Juliany Cf Rodrigues1,2,3, Luiz Augusto S de Oliveira1,4. 1. Núcleo Multidisciplinar de Pesquisas em Biologia, Campus Prof. Geraldo Cidade, Universidade Federal do Rio de Janeiro, Rodovia Washington Luiz, km 105. 25240-005, Duque de Caxias, RJ, Brazil. 2. Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem, Rio de Janeiro, RJ, 21941-902, Brazil. 3. Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, RJ, 21941-170, Brazil. 4. Núcleo Multidisciplinar de Pesquisas em Nanotecnologia, Campus Prof. Geraldo Cidade, Universidade Federal do Rio de Janeiro, Rodovia Washington Luiz, km 105. 25240-005, Duque de Caxias, RJ, Brazil. 5. Facultad de Física, IMRE, Universidad de La Habana, San Lazaro y L, C. Habana, CP 10400, Cuba. 6. Centro Brasileiro de Pesquisas Físicas, Rua Xavier Sigaud 150, Rio de Janeiro, RJ, 22290-180, Brazil.
Abstract
Aim: The primary goal of this work was to synthesize low-cost superparamagnetic iron oxide nanoparticles (SPIONs) with the aid of coconut water and evaluate the ability of macrophages to internalize them. Our motivation was to determine potential therapeutic applications in drug-delivery systems associated with magnetic hyperthermia. Materials & methods: We used the following characterization techniques: x-ray and electron diffractions, electron microscopy, spectrometry and magnetometry. Results: The synthesized SPIONs, roughly 4 nm in diameter, were internalized by macrophages, likely via endocytic/phagocytic pathways. They were randomly distributed throughout the cytoplasm and mainly located in membrane-bound compartments. Conclusion: Nanoparticles presented an elevated intrinsic loss power value and were not cytotoxic to mammalian cells. Thus, we suggest that low-cost SPIONs have great therapeutic potential.
Aim: The primary goal of this work was to synthesize low-cost superparamagnetic iron oxide nanoparticles (SPIONs) with the aid of coconut water and evaluate the ability of macrophages to internalize them. Our motivation was to determine potential therapeutic applications in drug-delivery systems associated with magnetic hyperthermia. Materials & methods: We used the following characterization techniques: x-ray and electron diffractions, electron microscopy, spectrometry and magnetometry. Results: The synthesized SPIONs, roughly 4 nm in diameter, were internalized by macrophages, likely via endocytic/phagocytic pathways. They were randomly distributed throughout the cytoplasm and mainly located in membrane-bound compartments. Conclusion: Nanoparticles presented an elevated intrinsic loss power value and were not cytotoxic to mammalian cells. Thus, we suggest that low-cost SPIONs have great therapeutic potential.
Entities:
Keywords:
biocompatibility; green synthesis; iron oxide nanoparticles; macrophages; magnetic hyperthermia; superparamagnetism
Authors: Silvia Soreto Teixeira; Manuel P F Graça; José Lucas; Manuel Almeida Valente; Paula I P Soares; Maria Carmo Lança; Tânia Vieira; Jorge Carvalho Silva; João Paulo Borges; Luiza-Izabela Jinga; Gabriel Socol; Cristiane Mello Salgueiro; José Nunes; Luís C Costa Journal: Nanomaterials (Basel) Date: 2021-01-14 Impact factor: 5.076