Literature DB >> 3141168

Enzyme immunoassay for the detection of Giardia lamblia.

J R Carlson1, P S Sullivan, D J Harry, M A Stork, S A Thorton, H L DuPont.   

Abstract

An enzyme immunoassay (EIA) was developed for direct detection of Giardia lamblia antigens in fecal specimens. The EIA was evaluated by testing specimens from 1,331 subjects in the USA and Egypt. For the 353 specimens from human subjects in the USA there was a 97% overall agreement between the results of the EIA and direct microscopic examination, yielding a sensitivity and specificity of 92% and 99% respectively. Due to adverse field conditions the EIA did not perform as well in the specimens collected and tested in Egypt. The sensitivity and specificity for 585 human specimens from Egypt were 74% and 97% respectively.

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Year:  1988        PMID： 3141168     DOI： 10.1007/bf01962610

Source DB:  PubMed          Journal:  Eur J Clin Microbiol Infect Dis        ISSN： 0934-9723            Impact factor:   3.267


  10 in total

Review 1.  Giardia and giardiasis.

Authors:  E A Meyer; S Radulescu
Journal:  Adv Parasitol       Date:  1979       Impact factor: 3.870

2.  Axenic culture of Giardia lamblia in TYI-S-33 medium supplemented with bile.

Authors:  D B Keister
Journal:  Trans R Soc Trop Med Hyg       Date:  1983       Impact factor: 2.184

3.  Immunodiagnostic detection of Giardia antigen in faeces by a rapid visual enzyme-linked immunosorbent assay.

Authors:  E L Green; M A Miles; D C Warhurst
Journal:  Lancet       Date:  1985-09-28       Impact factor: 79.321

4.  Ethyl acetate as a substitute for diethyl ether in the formalin-ether sedimentation technique.

Authors:  K H Young; S L Bullock; D M Melvin; C L Spruill
Journal:  J Clin Microbiol       Date:  1979-12       Impact factor: 5.948

5.  Diagnosis of giardiasis by counterimmunoelectrophoresis of feces.

Authors:  J C Craft; J D Nelson
Journal:  J Infect Dis       Date:  1982-04       Impact factor: 5.226

6.  Comparison of methods for identification of Giardia lamblia.

Authors:  S A Thornton; A H West; H L DuPont; L K Pickering
Journal:  Am J Clin Pathol       Date:  1983-12       Impact factor: 2.493

7.  Enzyme-linked immunosorbent assay for the detection of Giardia lamblia in fecal specimens.

Authors:  B L Ungar; R H Yolken; T E Nash; T C Quinn
Journal:  J Infect Dis       Date:  1984-01       Impact factor: 5.226

8.  Detection of Giardia lamblia antigen in the feces by counterimmunoelectrophoresis.

Authors:  V K Vinayak; K Kum; R Chandna; K Venkateswarlu; S Mehta
Journal:  Pediatr Infect Dis       Date:  1985 Jul-Aug

Review 9.  Giardia and giardiasis in childhood.

Authors:  J C Craft
Journal:  Pediatr Infect Dis       Date:  1982 May-Jun

10.  The detection of enteropathogens in acute diarrhea in a family cohort population in rural Egypt.

Authors:  A M Zaki; H L DuPont; M A el Alamy; R R Arafat; K Amin; M M Awad; L Bassiouni; I Z Imam; G S el Malih; A el Marsafie
Journal:  Am J Trop Med Hyg       Date:  1986-09       Impact factor: 2.345
  10 in total
  4 in total

1.  Laboratory diagnosis for Giardia lamblia infection: A comparison of microscopy, coprodiagnosis and serology.

Authors:  M A Behr; E Kokoskin; T W Gyorkos; L Cédilotte; G M Faubert; J D Maclean
Journal:  Can J Infect Dis       Date:  1997-01

2.  Purification and characterization of Giardia lamblia antigens in the feces of Mongolian gerbils.

Authors:  D M Moss; H M Mathews; G S Visvesvara; J W Dickerson; E M Walker
Journal:  J Clin Microbiol       Date:  1991-01       Impact factor: 5.948

3.  Evaluation of a commercially available enzyme-linked immunosorbent assay for Giardia lamblia antigen in stool.

Authors:  D G Addiss; H M Mathews; J M Stewart; S P Wahlquist; R M Williams; R J Finton; H C Spencer; D D Juranek
Journal:  J Clin Microbiol       Date:  1991-06       Impact factor: 5.948

4.  A novel, multi-parallel, real-time polymerase chain reaction approach for eight gastrointestinal parasites provides improved diagnostic capabilities to resource-limited at-risk populations.

Authors:  Rojelio Mejia; Yosselin Vicuña; Nely Broncano; Carlos Sandoval; Maritza Vaca; Martha Chico; Philip J Cooper; Thomas B Nutman
Journal:  Am J Trop Med Hyg       Date:  2013-03-18       Impact factor: 2.345
  4 in total

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