A dual-site controlled ratiometric probe revealing the simultaneous down-regulation of pH in lysosomes and cytoplasm during autophagy.

In this work, a unique dual-site controlled fluorescent probe was presented for the sensitive and concurrent detection of pH in the cytoplasm and lysosomes. With the probe, the simultaneous down-regulation of pH in the lysosomes and cytoplasm during autophagy has been successfully revealed for the first time.