Karl Stattin1, Lars Lind2, Sölve Elmståhl3, Alicja Wolk1,4, Eva Warensjö Lemming1, Håkan Melhus2, Karl Michaëlsson1, Liisa Byberg1. 1. Department of Surgical Sciences, Orthopaedics, Uppsala University, Sweden. 2. Department of Medical Sciences, Uppsala University, Sweden. 3. Department of Clinical Sciences, Division of Geriatric Medicine, Lund University, Sweden. 4. Unit of Cardiovascular and Nutritional Epidemiology, Institute for Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.
Abstract
AIMS: We aimed to discover and replicate associations between leisure-time physical activity and cardiovascular candidate plasma protein biomarkers and to examine whether the associations were independent of body fat. METHODS: We used cross-sectional data from two population-based cohorts, the EpiHealth (discovery cohort; n = 2239) and the Swedish Mammography Cohort - Clinical (SMCC; replication cohort; n = 4320). Physical activity during leisure time was assessed using questionnaires, and plasma concentrations of 184 proteins were assayed using the Olink Proseek Multiplex Cardiovascular 2 and 3 kits. We applied adjusted linear regression models using the False Discovery Rate to control for multiple testing in discovery. RESULTS: In EpiHealth, physical activity was associated with 75 cardiovascular plasma biomarkers, of which 28 associations were verified (replicated) in SMCC. Findings include seven novel associations in human: paraoxonase 3, cystatin B, cathepsin Z, alpha-L-iduronidase, prostasin, growth differentiation factor 2 and tumour necrosis factor alpha receptor superfamily member 11A. Estimates for associations were similar across tertiles of body fat and physical activity was associated with four biomarkers independent of body fat percentage: paraoxonase 3, cystatin B, fatty acid-binding protein 4 and interleukin-1 receptor antagonist. CONCLUSION: Leisure-time physical activity was associated with 28 cardiovascular-specific proteins; four associations were independent of body fat. Biomarkers in novel associations are involved in several atherosclerotic processes including regulation of low-density lipoprotein oxidation, protein degradation and immune cell adhesion and migration. Further research into these pathways may yield new insights into how physical activity affects cardiovascular health.
AIMS: We aimed to discover and replicate associations between leisure-time physical activity and cardiovascular candidate plasma protein biomarkers and to examine whether the associations were independent of body fat. METHODS: We used cross-sectional data from two population-based cohorts, the EpiHealth (discovery cohort; n = 2239) and the Swedish Mammography Cohort - Clinical (SMCC; replication cohort; n = 4320). Physical activity during leisure time was assessed using questionnaires, and plasma concentrations of 184 proteins were assayed using the Olink Proseek Multiplex Cardiovascular 2 and 3 kits. We applied adjusted linear regression models using the False Discovery Rate to control for multiple testing in discovery. RESULTS: In EpiHealth, physical activity was associated with 75 cardiovascular plasma biomarkers, of which 28 associations were verified (replicated) in SMCC. Findings include seven novel associations in human: paraoxonase 3, cystatin B, cathepsin Z, alpha-L-iduronidase, prostasin, growth differentiation factor 2 and tumour necrosis factor alpha receptor superfamily member 11A. Estimates for associations were similar across tertiles of body fat and physical activity was associated with four biomarkers independent of body fat percentage: paraoxonase 3, cystatin B, fatty acid-binding protein 4 and interleukin-1 receptor antagonist. CONCLUSION: Leisure-time physical activity was associated with 28 cardiovascular-specific proteins; four associations were independent of body fat. Biomarkers in novel associations are involved in several atherosclerotic processes including regulation of low-density lipoprotein oxidation, protein degradation and immune cell adhesion and migration. Further research into these pathways may yield new insights into how physical activity affects cardiovascular health.
Authors: Laura Corlin; Chunyu Liu; Honghuang Lin; Dominick Leone; Qiong Yang; Debby Ngo; Daniel Levy; L Adrienne Cupples; Robert E Gerszten; Martin G Larson; Ramachandran S Vasan Journal: J Am Heart Assoc Date: 2020-12-29 Impact factor: 5.501
Authors: Amir Zaghi; Hannes Holm; Johan Korduner; Anna Dieden; John Molvin; Erasmus Bachus; Amra Jujic; Martin Magnusson Journal: Front Cardiovasc Med Date: 2022-02-21
Authors: Grace Liang; Xianxi Huang; James Hirsch; Sanjeev Mehmi; Holly Fonda; Khin Chan; Ngan F Huang; Oliver Aalami; Victor F Froelicher; David P Lee; Jonathan Myers; Andrew S Lee; Patricia K Nguyen Journal: Front Cardiovasc Med Date: 2021-06-17