Oguz Ari1, Sema Karabudak1, M Tayyar Kalcioglu2, A Yasemin Gunduz3, Riza Durmaz4. 1. Ankara Yildirim Beyazit University, Central Research and Application Center, Ankara, Turkey. 2. Istanbul Medeniyet University, Faculty of Medicine, Goztepe Training and Research Hospital, Department of Otorhinolaryngology, Istanbul, Turkey. Electronic address: mahmut.kalcioglu@medeniyet.edu.tr. 3. Istanbul Medeniyet University, Faculty of Medicine, Goztepe Training and Research Hospital, Department of Otorhinolaryngology, Istanbul, Turkey. 4. Ankara Yildirim Beyazit University, Central Research and Application Center, Ankara, Turkey; Ankara Yildirim Beyazit University, Faculty of Medicine Department of Clinical Microbiology, Ankara, Turkey.
Abstract
OBJECTIVE: The aim of this study was to evaluate the composition and the diversity of bacteriome in middle ear effusion (MEE) and adenoid specimens of pediatric patients having otitis media with effusion (OME). MATERIALS AND METHODS: Sample collection from children with OME followed by next generation sequencing. Seventeen adenoid and 43 middle ear effusion specimens from 25 children having OME were evaluated. Microbiome analysis was performed via Ion 16S rRNA metagenomics kit. RESULTS: Twenty-two different bacterial species were identified from all of the samples analyzed. There were variations in the prevalence and relative abundance of the bacteriome observed between adenoid and MEE samples. MEE microbiome was significantly dominated by Alloicoccus otitis (44%), Turicella otitidis (6%), and Staphylococcus auricularis (3%). Whereas, Rothia mucilaginosa (39%), R. dentocariosa (11%), S. aureus (5%), Veillonella rogosae (2%), Granulicatella elegans (2%), Granulicatella adiacens (2%), Eikenella corrodens (1%), and Prevotella nanceiensis (1%) had significantly higher relative abundance in adenoid samples. Overall, there was no statistically significant difference in alpha diversity of MEE and adenoid samples, whereas adenoid samples constituted a cluster in the beta diversity graph. CONCLUSION: Bacteriome of MEE is mostly dominated by A. otitis yet accompanied by other bacteria with lower relative abundances suggests that OME is likely to be a polymicrobial process. Despite similarities, significant differences in relative abundances of several predominant species between bacteriome in the MEE and adenoid put the theory that OME in children is originated from the adenoids under question.
OBJECTIVE: The aim of this study was to evaluate the composition and the diversity of bacteriome in middle ear effusion (MEE) and adenoid specimens of pediatric patients having otitis media with effusion (OME). MATERIALS AND METHODS: Sample collection from children with OME followed by next generation sequencing. Seventeen adenoid and 43 middle ear effusion specimens from 25 children having OME were evaluated. Microbiome analysis was performed via Ion 16S rRNA metagenomics kit. RESULTS: Twenty-two different bacterial species were identified from all of the samples analyzed. There were variations in the prevalence and relative abundance of the bacteriome observed between adenoid and MEE samples. MEE microbiome was significantly dominated by Alloicoccus otitis (44%), Turicella otitidis (6%), and Staphylococcus auricularis (3%). Whereas, Rothia mucilaginosa (39%), R. dentocariosa (11%), S. aureus (5%), Veillonella rogosae (2%), Granulicatella elegans (2%), Granulicatella adiacens (2%), Eikenella corrodens (1%), and Prevotella nanceiensis (1%) had significantly higher relative abundance in adenoid samples. Overall, there was no statistically significant difference in alpha diversity of MEE and adenoid samples, whereas adenoid samples constituted a cluster in the beta diversity graph. CONCLUSION: Bacteriome of MEE is mostly dominated by A. otitis yet accompanied by other bacteria with lower relative abundances suggests that OME is likely to be a polymicrobial process. Despite similarities, significant differences in relative abundances of several predominant species between bacteriome in the MEE and adenoid put the theory that OME in children is originated from the adenoids under question.
Authors: Rıza Durmaz; Bengül Durmaz; Oğuz Arı; Olkar Abdulmajed; Serdal Çelik; Mahmut Tayyar Kalcıoğlu Journal: Turk Arch Otorhinolaryngol Date: 2022-02-22
Authors: Wei Cao; Yi Sun; Na Zhao; Jun Song; Nanfeng Zhang; Long Liu; Qian Liu Journal: Eur Arch Otorhinolaryngol Date: 2021-07-24 Impact factor: 2.503