| Literature DB >> 31407300 |
Samuel E Barnett1, Nicholas D Youngblut2, Daniel H Buckley3.
Abstract
DNA stable isotope probing (DNA-SIP) allows for the identification of microbes that assimilate isotopically labeled substrates into DNA. Here we describe the analysis of sequencing data using the multiple window high-resolution DNA-SIP method (MW-HR-SIP). MW-HR-SIP has improved accuracy over other methods and is easily implemented on the statistical platform R. We also discuss key experimental parameters to consider when designing DNA-SIP experiments and how these parameters affect accuracy of analysis.Entities:
Keywords: DNA-SIP; High throughput sequencing; R; Stable isotope probing
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Year: 2019 PMID： 31407300 DOI： 10.1007/978-1-4939-9721-3_9
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745