Zahra Sedaghat1, Hossein Fatemikia2,3, Kaveh Tanha4,5, Maria Zahiri6, Majid Assadi5. 1. Physiology Department, School of Medicine, Bushehr University of Medical Sciences, Moallem Street, Bushehr, 7514633341, Iran. z.sedaghat@bpums.ac.ir. 2. Physiology Department, School of Medicine, Bushehr University of Medical Sciences, Moallem Street, Bushehr, 7514633341, Iran. 3. Physiology Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. 4. Medical Physics and Biomedical Engineering Department, Tehran University of Medical Sciences, Tehran, Iran. 5. The Persian Gulf Nuclear Medicine Research Center, Bushehr University of Medical Sciences, Bushehr, Iran. 6. Department of Anatomical Sciences, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran.
Abstract
PURPOSE: To determine the role of remote perconditioning (RPeC) on renal function and histology in an animal model of unilateral renal ischemia and reperfusion (IR) injury. METHODS: Rats were subjected to 60 min unilateral renal ischemia. RPeC protocol was the application of four cycles of 5 min IR of left femoral artery during renal ischemia. Assessments of histological changes and renal function were made 24 h, 1 week, or 3 weeks later. 99mTc-DMSA scan was performed using a small-animals SPECT system. RESULTS: 24-h reperfusion decreased the 99mTc-DMSA uptake in the left kidney compared to the intact kidney of control animals. RPeC group has higher uptake compared to the IR group. After 1 week and 3 weeks, uptakes were gradually increased in both groups and no differences were observed. Severe morphological changes in the ischemic kidneys of both groups were observed after 24 h which attenuated after 1 week and 3 weeks. Moreover, no differences in creatinine and BUN levels between IR-treated and intact animals were observed. CONCLUSION: These data suggest that RPeC exerts a partially transient improvement in the renal function in the first day after reperfusion. However, long-term follow-up study showed no beneficial effects of RPeC. Moreover, noninvasive 99mTc-DMSA scan revealed a suitable tool in the follow-up evaluation of recovery process in the unilateral renal IR injury models.
PURPOSE: To determine the role of remote perconditioning (RPeC) on renal function and histology in an animal model of unilateral renal ischemia and reperfusion (IR) injury. METHODS:Rats were subjected to 60 min unilateral renal ischemia. RPeC protocol was the application of four cycles of 5 min IR of left femoral artery during renal ischemia. Assessments of histological changes and renal function were made 24 h, 1 week, or 3 weeks later. 99mTc-DMSA scan was performed using a small-animals SPECT system. RESULTS: 24-h reperfusion decreased the 99mTc-DMSA uptake in the left kidney compared to the intact kidney of control animals. RPeC group has higher uptake compared to the IR group. After 1 week and 3 weeks, uptakes were gradually increased in both groups and no differences were observed. Severe morphological changes in the ischemic kidneys of both groups were observed after 24 h which attenuated after 1 week and 3 weeks. Moreover, no differences in creatinine and BUN levels between IR-treated and intact animals were observed. CONCLUSION: These data suggest that RPeC exerts a partially transient improvement in the renal function in the first day after reperfusion. However, long-term follow-up study showed no beneficial effects of RPeC. Moreover, noninvasive 99mTc-DMSA scan revealed a suitable tool in the follow-up evaluation of recovery process in the unilateral renal IR injury models.
Authors: Ola Z Ismail; Xizhong Zhang; Junjun Wei; Aaron Haig; Bradley M Denker; Rita S Suri; Alp Sener; Lakshman Gunaratnam Journal: Am J Pathol Date: 2015-03-07 Impact factor: 4.307
Authors: Marie Louise V Kristensen; Casper Kierulf-Lassen; Per Mose Nielsen; Søren Krag; Henrik Birn; Lene N Nejsum; Rikke Nørregaard Journal: Physiol Rep Date: 2016-07